公开(公告)号：US09797004B2

公开(公告)日：2017-10-24

申请号：US13913937

申请日：2013-06-10

Applicant: Syngenta Participations AG

Inventor： Chengkun He ,  Chunyang Fan ,  John Ke ,  Heng Zhong ,  Doug Russell

IPC: C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/6848 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8209 ,  C12Q1/6806 ,  C12Q2600/166 ,  C12Q2537/165

Abstract: The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.

公开(公告)号：US20130330733A1

公开(公告)日：2013-12-12

申请号：US13913937

申请日：2013-06-10

Applicant: Syngenta Participations AG

Inventor： Chengkun He ,  Chunyang Fan ,  John Ke ,  Heng Zhong ,  Doug Russell

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8209 ,  C12Q1/6806 ,  C12Q2600/166 ,  C12Q2537/165

Abstract: The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.

Abstract translation: 本发明提供用于在包括感兴趣多核苷酸的核酸制备物中检测和/或定量载体骨架的方法和组合物，所述扩增测定法在可以发生扩增的条件下扩增位于所述多核苷酸和载体骨架之间的连接，其中 连接点包含核酸酶的识别位点，并且检测不存在扩增产物，由此不存在扩增产物指示低或无载体骨架和/或定量扩增产物的量以确定载体骨架的量 核酸制剂。