公开(公告)号：US20180092316A1

公开(公告)日：2018-04-05

申请号：US15354912

申请日：2016-11-17

Applicant: Syngenta Participations AG

Inventor： Satya CHINTAMANANI ,  Timothy KELLIHER ,  Brent DELZER ,  Michael NUCCIO ,  Robert Arthur DIETRICH ,  Suresh Babu KADARU ,  Todd WARNER ,  William Paul BULLOCK

IPC: A01H1/08 ,  A01H5/10 ,  C12Q1/6895 ,  A01H1/04 ,  C12N15/82

CPC classification number: A01H1/08 ,  A01H1/04 ,  A01H5/10 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8287 ,  C12N2310/14 ,  C12N2310/20 ,  C12Q1/6895 ,  C12Y301/01002 ,  Y02A40/146

Abstract: Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.