公开(公告)号：US20180092316A1

公开(公告)日：2018-04-05

申请号：US15354912

申请日：2016-11-17

Applicant: Syngenta Participations AG

Inventor： Satya CHINTAMANANI ,  Timothy KELLIHER ,  Brent DELZER ,  Michael NUCCIO ,  Robert Arthur DIETRICH ,  Suresh Babu KADARU ,  Todd WARNER ,  William Paul BULLOCK

IPC: A01H1/08 ,  A01H5/10 ,  C12Q1/6895 ,  A01H1/04 ,  C12N15/82

CPC classification number: A01H1/08 ,  A01H1/04 ,  A01H5/10 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8287 ,  C12N2310/14 ,  C12N2310/20 ,  C12Q1/6895 ,  C12Y301/01002 ,  Y02A40/146

Abstract: Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

公开(公告)号：US20170067067A1

公开(公告)日：2017-03-09

申请号：US14212504

申请日：2014-03-14

Applicant: SYNGENTA PARTICIPATIONS AG

Inventor： Satya CHINTAMANANI ,  Brent DELZER ,  Michael L NUCCIO ,  Robert Arthur DIETRICH ,  Suresh Babu KADARU ,  Todd Lee WARNER ,  William Paul BULLOCK ,  Timothy KELLIHER

IPC: C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8216 ,  A01H1/04 ,  A01H1/08 ,  C12N9/18 ,  C12N15/8218 ,  C12N15/8261 ,  C12N15/8287 ,  C12Q1/686 ,  C12Q1/6895 ,  Y02A40/146

Abstract: Provided are isolated cDNAs. In some embodiments, the isolated cDNA are selected from the group consisting of: (a) a nucleic acid having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53, optionally wherein the percent identity is calculated over the entire length of SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53; (b) a nucleic acid having at least 95% identity over nucleotides 91-1452 of SEQ ID NO: 33; (c) a nucleic acid that is the reverse complement of either of (a) or (b); and (d) a nucleic acid that encodes a polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 54, or SEQ ID NO: 55, or SEQ ID NO: 56, or SEQ ID NO: 57 optionally wherein the percent identity is calculated over the entire length of SEQ ID NO: 54, or SEQ ID NO: 55, or SEQ ID NO: 56, or SEQ ID NO: 57.Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid—inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Abstract translation: 还提供了表达盒; 向量 转基因植物细胞; 植物，植物部分和种子; 分离的多肽; 目前公开的核酸的扩增子和信息片段; 包括扩增引物对的组合物; 生产展示HI的植物的方法; 用于鉴定植物中与HI相关的等位基因的存在或不存在的方法; 将单倍体诱导核苷酸序列插入植物的方法; 以及用于选择预期用表现单倍体诱导性状的植物产生后代的亲本植物的方法。

公开(公告)号：US20180332790A1

公开(公告)日：2018-11-22

申请号：US15776957

申请日：2016-11-17

Applicant: Syngenta Participations AG

Inventor： Timothy KELLIHER ,  Brent DELZER ,  Satya CHINTAMANANI ,  David Stewart SKIBBE ,  Zhongying CHEN ,  Dakota STARR ,  Sebastian Volker WENDEBORN ,  Mark LEDSON ,  Jeffrey David FOWLER

IPC: A01H1/08

CPC classification number: A01H1/08 ,  C07K14/415 ,  C12N9/18 ,  C12N15/8261 ,  C12N15/8287

Abstract: Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

公开(公告)号：US20180273963A1

公开(公告)日：2018-09-27

申请号：US15901464

申请日：2018-02-21

Applicant: Syngenta Participations AG

Inventor： Timothy KELLIHER ,  Qiudeng QUE

IPC: C12N15/82 ,  C12Q1/6895

CPC classification number: C12N15/8216 ,  A01H1/08 ,  C07K14/415 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8287 ,  C12Q1/6895 ,  C12Y301/01032

Abstract: The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.