公开(公告)号：US20170166871A1

公开(公告)日：2017-06-15

申请号：US15323811

申请日：2015-07-09

申请人： TAKARA BIO INC.

发明人： Toshikazu NISHIE ,  Yasuhiro KAWANO ,  Shuhei SAKAMOTO ,  Tatsuji ENOKI ,  Hikaru TAKAKURA ,  Junichi MINENO

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N7/02 ,  C12N2750/14151

摘要： Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.

公开(公告)号：US20160152955A1

公开(公告)日：2016-06-02

申请号：US14900837

申请日：2014-07-10

申请人： TAKARA BIO INC.

发明人： Shuhei SAKAMOTO ,  Yasuhiro KAWANO ,  Katsuyuki DODO ,  Tatsuji ENOKI ,  Hirofumi YOSHIOKA ,  Hikaru TAKAKURA ,  Junichi MINENO

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N2750/14151 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2750/14043 ,  C12N2750/14122 ,  C12N2750/14142 ,  C12N7/02 ,  C12N2750/14141 ,  C12N2750/14351 ,  C12N2800/50 ,  C12N15/8673 ,  C12N2750/14352 ,  C12N2750/14152

摘要： The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.

摘要翻译： 本发明提供了通过培养具有产生非包膜病毒的能力的细胞并使细胞和酸性溶液相互接触的方式，高效地制造无包膜病毒的方法，而无需费力的操作。 通过本发明方法制备的非包膜病毒载体和具有非包膜病毒载体作为活性成分的组合物在基础研究和临床应用基因治疗领域中作为基因转移方法是非常有用的。