公开(公告)号：US20210222137A1

公开(公告)日：2021-07-22

申请号：US17257986

申请日：2019-07-03

申请人： TAKARA BIO INC.

发明人： Takashi UEMORI ,  Hiroyuki MATSUMOTO ,  Kensuke SAITO ,  Miwa AKITOMO

IPC分类号： C12N9/12

摘要： Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

公开(公告)号：US20230160023A1

公开(公告)日：2023-05-25

申请号：US17921451

申请日：2021-04-28

申请人： TAKARA BIO INC.

发明人： Takashi UEMORI ,  Takehiro SAGARA ,  Miwa AKITOMO ,  Kensuke SAITO

IPC分类号： C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12N9/12 ,  C12N9/50

CPC分类号： C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12N9/1252 ,  C12N9/50 ,  C12N2320/10

摘要： Provided is a method for detecting an FNA virus in a biological sample, the method comprising: (1) a step for preparing a sample solution containing a biological sample, a protease, a nucleic acid that does not become a target of nucleic acid amplification, and at least one additive selected from the group consisting of chaotropic reagents and surfactants; (2) a step for preparing a nucleic acid amplification reaction solution containing the sample solution prepared in step (1) and containing a polypeptide having reverse transcriptase activity and DMA polymerase activity or a polypeptide having reverse transcription activity and a polypeptide having DNA polymerase activity; and (3) a step for amplifying a nucleic acid of the RNA virus in the reaction solution prepared in step (2).

公开(公告)号：US20210164018A1

公开(公告)日：2021-06-03

申请号：US16616532

申请日：2018-06-06

申请人： TAKARA BIO INC.

发明人： Miwa AKITOMO ,  Takashi UEMORI

IPC分类号： C12Q1/6806 ,  C07K14/245

摘要： The present invention pertains to: an oligonucleotide preservation method; and a kit comprising an oligonucleotide. The present invention provides a method for stably preserving an oligonucleotide-containing solution by adding a nucleic acid-binding protein to said oligonucleotide-containing solution in advance.