公开(公告)号：US20230287350A1

公开(公告)日：2023-09-14

申请号：US18005879

申请日：2021-08-16

Applicant: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD

Inventor： Shulamit Levenberg ,  Shaowei Guo ,  Lior Debbi

IPC: C12N5/077 ,  C12M1/42 ,  C12M1/12

CPC classification number: C12N5/0658 ,  C12M35/04 ,  C12M25/14 ,  C12N2513/00

Abstract: The present invention provides methods and systems for enhanced production and/or secretion of extracellular vesicles (EVs) from muscle cells utilizing various dynamic mechanical loading profiles thereon, cultured on three-dimensional (3D) scaffolds. The scaffolds may comprise a plurality of layers, wherein each layer comprises a plurality of elastic microfibers, and wherein the microfibers are aligned in parallel to a longitudinal axis and to each other. The elastic 3D scaffold may be configured to undergo dynamic mechanical loading profiles and support an expansion of a population of muscle cells cultured thereon into a 3D multi-layer structure of muscle cells, wherein said 3D multi-layer structure is configured to produce and/or secret extracellular vesicles into a medium.