公开(公告)号：US20230287350A1

公开(公告)日：2023-09-14

申请号：US18005879

申请日：2021-08-16

Applicant: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD

Inventor： Shulamit Levenberg ,  Shaowei Guo ,  Lior Debbi

IPC: C12N5/077 ,  C12M1/42 ,  C12M1/12

CPC classification number: C12N5/0658 ,  C12M35/04 ,  C12M25/14 ,  C12N2513/00

Abstract: The present invention provides methods and systems for enhanced production and/or secretion of extracellular vesicles (EVs) from muscle cells utilizing various dynamic mechanical loading profiles thereon, cultured on three-dimensional (3D) scaffolds. The scaffolds may comprise a plurality of layers, wherein each layer comprises a plurality of elastic microfibers, and wherein the microfibers are aligned in parallel to a longitudinal axis and to each other. The elastic 3D scaffold may be configured to undergo dynamic mechanical loading profiles and support an expansion of a population of muscle cells cultured thereon into a 3D multi-layer structure of muscle cells, wherein said 3D multi-layer structure is configured to produce and/or secret extracellular vesicles into a medium.

公开(公告)号：US09718057B2

公开(公告)日：2017-08-01

申请号：US14760589

申请日：2014-01-15

Applicant: Technion Research and Development Foundation LTD.

Inventor： Shulamit Levenberg ,  Jonathan Shemesh

IPC: B01L3/00 ,  G01N1/30 ,  G01N1/18 ,  C40B50/00

CPC classification number: B01L3/502723 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/0694 ,  B01L2200/16 ,  B01L2300/048 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2400/0406 ,  G01N1/18 ,  G01N1/30 ,  Y10T436/2575

Abstract: A microfluidic device includes a platform with a microstructure. The microstructure include a primary channel and a plurality of chambers that open to the primary channel to enable a sample fluid that is loaded into the device via the primary channel to flow into the chambers. Each chamber has a volume that is less than tens of nanoliters and is connected by a vent to a secondary channel of the microstructure. A width of the vent is configured to enable a gas to escape from the chamber to the secondary channel while inhibiting flow of the sample fluid from the chamber into the secondary channel.

公开(公告)号：US20150352552A1

公开(公告)日：2015-12-10

申请号：US14760589

申请日：2014-01-15

Applicant: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

Inventor： Shulamit Levenberg ,  Jonathan Shemesh

IPC: B01L3/00 ,  G01N1/30

CPC classification number: B01L3/502723 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/0694 ,  B01L2200/16 ,  B01L2300/048 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2400/0406 ,  G01N1/18 ,  G01N1/30 ,  Y10T436/2575

Abstract: A microfluidic device includes a platform with a microstructure. The microstructure include a primary channel and a plurality of chambers that open to the primary channel to enable a sample fluid that is loaded into the device via the primary channel to flow into the chambers. Each chamber has a volume that is less than tens of nanoliters and is connected by a vent to a secondary channel of the microstructure. A width of the vent is configured to enable a gas to escape from the chamber to the secondary channel while inhibiting flow of the sample fluid from the chamber into the secondary channel.

Abstract translation: 微流体装置包括具有微结构的平台。 微结构包括主通道和多个腔室，其通向主通道，以使经由主通道加载到设备中的样品流体流入腔室。 每个室具有小于数十纳升的体积，并且通过排气口连接到微结构的第二通道。 排气口的宽度被配置成使得气体能够从腔室逸出到次级通道，同时抑制样品流体从腔室流入第二通道。