公开(公告)号：US20200041451A1

公开(公告)日：2020-02-06

申请号：US16339348

申请日：2017-10-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Noel Seunghyun Ha ,  R. Michael Van Dam ,  Jimmy Ly

IPC: G01N27/447 ,  B01L3/00 ,  G01N27/453

Abstract: A volumetric microfluidic injector for capillary electrophoresis (CE) for highly repeatable sample injection has been designed and built to eliminate known injection bias in hydrodynamic injection. A defined volume from 1-10 nL or 0.1-100 nL of sample is confined in a defined region of a micro-valve PDMS microfluidic injector chip and electrophoretic potential is applied to drive sample into a separation device such as an embedded fused silica capillary for separation and detection. Using a 75 μm ID capillary, the RSD of an absorbance peak area as low as 1.32% (n=11) is obtained. As a comparison, the time-dependent injection was tested using the same chip which resulted in an inferior repeatability.

公开(公告)号：US11209390B2

公开(公告)日：2021-12-28

申请号：US16339348

申请日：2017-10-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Noel Seunghyun Ha ,  R. Michael Van Dam ,  Jimmy Ly

IPC: G01N27/447 ,  B01L3/00 ,  G01N27/453

Abstract: A volumetric microfluidic injector for capillary electrophoresis (CE) for highly repeatable sample injection has been designed and built to eliminate known injection bias in hydrodynamic injection. A defined volume from 1-10 nL or 0.1-100 nL of sample is confined in a defined region of a micro-valve PDMS microfluidic injector chip and electrophoretic potential is applied to drive sample into a separation device such as an embedded fused silica capillary for separation and detection. Using a 75 μm ID capillary, the RSD of an absorbance peak area as low as 1.32% (n=11) is obtained. As a comparison, the time-dependent injection was tested using the same chip which resulted in an inferior repeatability.