公开(公告)号：US20160123964A1

公开(公告)日：2016-05-05

申请号：US14933853

申请日：2015-11-05

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Paul C. TUMEH ,  Antoni RIBAS

IPC: G01N33/50 ,  A61K39/395 ,  A61K35/15 ,  C07K16/28

CPC classification number: G01N33/5091 ,  A61K2039/505 ,  C07K16/2818 ,  C07K2317/76 ,  G01N2800/52

Abstract: A method of analyzing a biological sample from a subject that has a tumor or cancer, comprising: determining, for target cells having a phenotype of interest spatial resolution of the target cells, density of the spatially resolved target cells in the sample; and proximity between spatially resolved target cells of interest in the sample; and determining an overall score based at least in part on the preceding parameters. A method for identifying a patient as a responder to single agent anti-PD-1 or anti-PD-L1 therapy is provided. Similar methods are provided for detecting adaptive immune resistance, the presence of cancer in a patient sample, determining efficacy of cancer therapy, and determining response to and monitoring the efficacy of cancer therapy.

Abstract translation: 一种从具有肿瘤或癌症的受试者分析生物样品的方法，包括：对于具有目标细胞的感兴趣空间分辨率的靶细胞，确定样品中空间分辨的靶细胞的密度; 以及样品中感兴趣的空间分辨的靶细胞之间的接近度; 以及至少部分地基于前述参数来确定总分。 提供了将患者识别为单剂抗PD-1或抗PD-L1治疗的应答​​者的方法。 提供了类似的方法用于检测适应性免疫耐受性，患者样品中存在癌症，确定癌症治疗的功效，以及确定对癌症治疗的疗效的响应和监测。