公开(公告)号：US11680907B2

公开(公告)日：2023-06-20

申请号：US17822734

申请日：2022-08-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ya-Hong Xie ,  Owen Liang

IPC: G01N33/574 ,  G01N21/65 ,  G01J3/02 ,  G01J3/44 ,  G01N33/569 ,  G01N33/68 ,  G01N21/35

CPC classification number: G01N21/658 ,  G01J3/0275 ,  G01J3/4412 ,  G01N33/56911 ,  G01N33/574 ,  G01N33/6803 ,  G01N33/6893 ,  G01J3/0264 ,  G01N2021/3595

Abstract: Systems and methods for characterizing biological specimens, which may involve identifying a cell type or state corresponding to a disease or health condition of a subject. A biological specimen is subjected to electromagnetic radiation for spectroscopic analysis such as Surface Enhanced Raman Spectroscopy (SERS) to determine the relative abundance of proteins or amino acids in the cells, which is used in a comparison to previously stored relative abundance data of a database to automatically identifies at least one of cell type and/or cell state of the cells (or the disease/health state of the subject with the disease state including the possibility of virus infection, or drug susceptibility of a subject to bacteria or fungus). The method may also be employed with biological entities or cellular structures such as exosomes and even protein or nucleic acid fragments to determine disease states or health states of the subject.

公开(公告)号：US11428638B2

公开(公告)日：2022-08-30

申请号：US16961675

申请日：2019-01-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ya-Hong Xie ,  Owen Liang

IPC: G01N33/574 ,  G01N21/65 ,  G01J3/02 ,  G01J3/44 ,  G01N33/569 ,  G01N33/68 ,  G01N21/35

Abstract: Systems and methods for characterizing biological specimens, which may involve identifying a cell type or state corresponding to a disease or health condition of a subject. A biological specimen is subjected to electromagnetic radiation for spectroscopic analysis such as Surface Enhanced Raman Spectroscopy (SERS) to determine the relative abundance of proteins or amino acids in the cells, which is used in a comparison to previously stored relative abundance data of a database to automatically identifies at least one of cell type and/or cell state of the cells (or the disease/health state of the subject with the disease state including the possibility of virus infection, or drug susceptibility of a subject to bacteria or fungus). The method may also be employed with biological entities or cellular structures such as exosomes and even protein or nucleic acid fragments to determine disease states or health states of the subject.

公开(公告)号：US20210372933A1

公开(公告)日：2021-12-02

申请号：US16961675

申请日：2019-01-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ya-Hong Xie ,  Owen Liang

IPC: G01N21/65 ,  G01J3/02 ,  G01N33/569 ,  G01N33/574 ,  G01N33/68 ,  G01J3/44

Abstract: Systems and methods for characterizing biological specimens, which may involve identifying a cell type or state corresponding to a disease or health condition of a subject. A biological specimen is subjected to electromagnetic radiation for spectroscopic analysis such as Surface Enhanced Raman Spectroscopy (SERS) to determine the relative abundance of proteins or amino acids in the cells, which is used in a comparison to previously stored relative abundance data of a database to automatically identifies at least one of cell type and/or cell state of the cells (or the disease/health state of the subject with the disease state including the possibility of virus infection, or drug susceptibility of a subject to bacteria or fungus). The method may also be employed with biological entities or cellular structures such as exosomes and even protein or nucleic acid fragments to determine disease states or health states of the subject.

公开(公告)号：US20220412892A1

公开(公告)日：2022-12-29

申请号：US17822734

申请日：2022-08-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ya-Hong Xie ,  Owen Liang

IPC: G01N21/65 ,  G01J3/02 ,  G01J3/44 ,  G01N33/569 ,  G01N33/574 ,  G01N33/68

Abstract: Systems and methods for characterizing biological specimens, which may involve identifying a cell type or state corresponding to a disease or health condition of a subject. A biological specimen is subjected to electromagnetic radiation for spectroscopic analysis such as Surface Enhanced Raman Spectroscopy (SERS) to determine the relative abundance of proteins or amino acids in the cells, which is used in a comparison to previously stored relative abundance data of a database to automatically identifies at least one of cell type and/or cell state of the cells (or the disease/health state of the subject with the disease state including the possibility of virus infection, or drug susceptibility of a subject to bacteria or fungus). The method may also be employed with biological entities or cellular structures such as exosomes and even protein or nucleic acid fragments to determine disease states or health states of the subject.

公开(公告)号：US10001442B2

公开(公告)日：2018-06-19

申请号：US14304290

申请日：2014-06-13

Applicant: The Regents of the University of California

Inventor： Ya-Hong Xie ,  Pu Wang

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01N21/658 ,  G01N2021/656

Abstract: An optical probe includes an optical fiber with a first end and a second end, and an enhanced surface on a portion of the first end of the optical fiber. The enhanced surface includes a patterned base layer including multiple protruding nano-pyramids, an intermediate layer over the patterned base layer, and a graphene layer over the intermediate layer. Using a layer of graphene to cover the enhanced surface increases the sensitivity of a surface-enhanced Raman spectroscopy (SERS) process performed in conjunction with the enhanced surface, and further increases the chemical stability and bio-compatibility of the enhanced surface. Further, placing the enhanced surface at the end of the optical fiber provides a self-contained probe for use with a SERS process, thereby allowing for in-vivo characterization of a sample.

公开(公告)号：US20140368817A1

公开(公告)日：2014-12-18

申请号：US14304290

申请日：2014-06-13

Applicant: The Regents of the University of California

Inventor： Ya-Hong Xie ,  Pu Wang

IPC: G01N21/65 ,  G01J3/44

CPC classification number: G01N21/658 ,  G01N2021/656

Abstract: An optical probe includes an optical fiber with a first end and a second end, and an enhanced surface on a portion of the first end of the optical fiber. The enhanced surface includes a patterned base layer including multiple protruding nano-pyramids, an intermediate layer over the patterned base layer, and a graphene layer over the intermediate layer. Using a layer of graphene to cover the enhanced surface increases the sensitivity of a surface-enhanced Raman spectroscopy (SERS) process performed in conjunction with the enhanced surface, and further increases the chemical stability and bio-compatibility of the enhanced surface. Further, placing the enhanced surface at the end of the optical fiber provides a self-contained probe for use with a SERS process, thereby allowing for in-vivo characterization of a sample.

Abstract translation: 光学探头包括具有第一端和第二端的光纤，以及在光纤的第一端的一部分上的增强表面。 增强表面包括图案化基底层，包括多个突出的纳米金字塔，在图案化基底层上方的中间层，以及中间层之上的石墨烯层。 使用一层石墨烯覆盖增强的表面增加了与增强表面结合进行的表面增强拉曼光谱（SERS）工艺的灵敏度，并进一步提高了增强表面的化学稳定性和生物相容性。 此外，将增强的表面放置在光纤末端提供了与SERS过程一起使用的独立探针，从而允许样品的体内表征。