公开(公告)号：US11209427B2

公开(公告)日：2021-12-28

申请号：US16498312

申请日：2018-03-26

Applicant: The Regents of the University of California

Inventor： Daniel T. Kamei ,  Maie A. ST John ,  Zachary D. Taylor ,  Daniel W. Bradbury ,  Ashley E. Kita

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/48 ,  H04M1/725

Abstract: Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (betaTP) and an indicator attached to a betaTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone.

公开(公告)号：US20190293789A1

公开(公告)日：2019-09-26

申请号：US16373777

申请日：2019-04-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Warren S. Grundfest ,  Maie St. John ,  Ashkan Maccabi ,  George Saddik ,  Zachary D. Taylor

IPC: G01S15/89 ,  G01S7/52 ,  G01S7/539 ,  B06B1/06 ,  A61B8/00

Abstract: A vibro-acoustography imaging system that that generates a map of the mechanical response of a target to an acoustic radiation force, usually in low kHz range by a confocal geometry. The system generates two focused sinusoidal beams to produce a stress field at the beat frequency, which is a function of vibration and acoustic emissions field in terms of mechanical properties. A highly sensitive hydrophone is then used for detection of the acoustic emissions field, the amplitude of which may be correlated to the mechanical properties of the target tissue.

公开(公告)号：US11867693B2

公开(公告)日：2024-01-09

申请号：US17530336

申请日：2021-11-18

Applicant: The Regents of the University of California

Inventor： Daniel Takashi Kamei ,  Maie A. St. John ,  Zachary D. Taylor ,  Daniel William Bradbury ,  Ashley Elizabeth Kita

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54386 ,  B01L3/5023 ,  B01L2400/0406 ,  G01N2333/99

Abstract: Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (βTP) and an indicator attached to a βTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone; and wherein said first test line (T1) and said second test line (T2) are is configured so that either no test line signal or just a signal at the first test line (T1) is detectable when βTP concentration in a sample applied to said device is lower than the βTP level indicative of a CSF leak; and said second test line (T2) is configured so that a signal is detectable at said second test line when βTP concentration in a sample applied to said device is greater than the βTP level indicative of a CSF leak.

公开(公告)号：US11857373B2

公开(公告)日：2024-01-02

申请号：US16373777

申请日：2019-04-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Warren S. Grundfest ,  Maie St. John ,  Ashkan Maccabi ,  George Saddik ,  Zachary D. Taylor

IPC: A61B8/08 ,  A61B8/00 ,  B06B1/06 ,  G01S15/89 ,  G01S7/52

CPC classification number: A61B8/485 ,  A61B8/085 ,  A61B8/4494 ,  B06B1/0622 ,  G01S7/52038 ,  G01S15/8913 ,  G01S15/8922 ,  G01S15/8952 ,  A61B8/5207 ,  G01S7/5203

Abstract: A vibro-acoustography imaging system that generates a map of the mechanical response of a target to an acoustic radiation force, usually in low kHz range by a confocal geometry. The system generates two focused sinusoidal beams to produce a stress field at the beat frequency, which is a function of vibration and acoustic emissions field in terms of mechanical properties. A highly sensitive hydrophone is then used for detection of the acoustic emissions field, the amplitude of which may be correlated to the mechanical properties of the target tissue.

公开(公告)号：US20220146507A1

公开(公告)日：2022-05-12

申请号：US17530336

申请日：2021-11-18

Applicant: The Regents of the University of California

Inventor： Daniel Takashi Kamei ,  Maie A. St. John ,  Zachary D. Taylor ,  Daniel William Bradbury ,  Ashley Elizabeth Kita

IPC: G01N33/543 ,  B01L3/00

Abstract: Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (βTP) and an indicator attached to a βTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone; and wherein said first test line (T1) and said second test line (T2) are is configured so that either no test line signal or just a signal at the first test line (T1) is detectable when βTP concentration in a sample applied to said device is lower than the βTP level indicative of a CSF leak; and said second test line (T2) is configured so that a signal is detectable at said second test line when βTP concentration in a sample applied to said device is greater than the βTP level indicative of a CSF leak.

公开(公告)号：US20200033336A1

公开(公告)日：2020-01-30

申请号：US16498312

申请日：2018-03-26

Applicant: The Regents of the University of California

Inventor： Daniel T. Kamei ,  Maie A. ST John ,  Zachary D. Taylor ,  Daniel W. Bradbury ,  Ashley E. Kita

IPC: G01N33/543 ,  B01L3/00

Abstract: Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (betaTP) and an indicator attached to a betaTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone.