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公开(公告)号：US11885803B2
公开(公告)日：2024-01-30
申请号：US17673530
申请日：2022-02-16
IPC分类号： G01N33/569 , G01N33/543 , G01N33/483 , G01N33/49 , G01N33/493
CPC分类号： G01N33/56927 , G01N33/4833 , G01N33/49 , G01N33/493 , G01N33/54388 , G01N33/54391 , G01N33/569 , G01N33/56944 , G01N33/56961 , G01N33/56983
摘要： In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6×106 cells/mL, less than about 3×106 cells/ml, less than about 1×106 CFU/mL, or less than about 50 μg/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.
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公开(公告)号：US20200150116A1
公开(公告)日：2020-05-14
申请号：US16616923
申请日：2018-05-30
发明人： Daniel Takashi Kamei , Benjamin Ming Wu , Garrett L. Mosley , Yin To Chiu , David Yuan Pereira , Chloe Michelle Wu , Yue Han , So Youn Lee
IPC分类号： G01N33/558 , G01N33/569
摘要： In various embodiments single-step ATPS paper-based diagnostic assays are provided that exploit the concept of sequential resolubilization of ATPS components to give rise to the desired phase separation behavior within paper. In one illustrative embodiment, a wick is provided for concentrating an analyte within an aqueous two-phase extraction system in a paper, where the wick comprises a paper configured to receive a sample where the paper comprises a first region containing a first component of an aqueous two-phase system (ATPS) where the first component is in a dry form, and a second region containing a second component of an aqueous two-phase system (ATPS) where the second component is in a dry form; and where said first region and the second region are disposed so that when said wick is contacted with a fluid sample, the first component of said ATPS is hydrated before the second component. In certain embodiments the first and second component are disposed so they are hydrated substantially simultaneously.
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公开(公告)号：US11635432B2
公开(公告)日：2023-04-25
申请号：US16751114
申请日：2020-01-23
IPC分类号： G01N33/558 , G01N33/543
摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
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公开(公告)号：US10578616B2
公开(公告)日：2020-03-03
申请号：US16434100
申请日：2019-06-06
IPC分类号： G01N33/558 , G01N33/543
摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
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5.发明申请公开(公告)号：US20190187140A1
公开(公告)日：2019-06-20
申请号：US16326687
申请日：2017-08-21
发明人： Daniel Takashi Kamei , Benjamin Ming Wu , David Yuan Pereira , Chloe Michelle Wu , Matthew Foosing Yee , Joshua Anthony Keefe , Christina Caroline Pearce , Amir Adam Dailamy , Vincent K. Wong , Nguyen Khoi Le
IPC分类号： G01N33/559 , G01N33/543 , G01N33/545 , G01N33/569 , G01N33/487
CPC分类号： G01N33/559 , C08J3/075 , C08J2333/14 , G01N1/28 , G01N33/48792 , G01N33/54353 , G01N33/5436 , G01N33/545 , G01N33/558 , G01N33/56983 , G01N2333/183 , G16H30/40
摘要： It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.
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公开(公告)号：US11828755B2
公开(公告)日：2023-11-28
申请号：US16307125
申请日：2017-06-07
发明人： Daniel Takashi Kamei , Benjamin Ming Wu , Daniel William Bradbury , Shin Ting Sherine Frieda Cheung
IPC分类号： G01N33/543 , C12Q1/6844 , C12Q1/6813
CPC分类号： G01N33/54388 , C12Q1/6813 , C12Q1/6844 , G01N33/543 , G01N33/54306 , C12Q1/6813 , C12Q2563/159 , C12Q2565/625 , C12Q1/6844 , C12Q2521/513 , C12Q2531/119 , C12Q2531/143 , C12Q2563/159
摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.
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公开(公告)号：US20220146507A1
公开(公告)日：2022-05-12
申请号：US17530336
申请日：2021-11-18
发明人： Daniel Takashi Kamei , Maie A. St. John , Zachary D. Taylor , Daniel William Bradbury , Ashley Elizabeth Kita
IPC分类号： G01N33/543 , B01L3/00
摘要： Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (βTP) and an indicator attached to a βTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone; and wherein said first test line (T1) and said second test line (T2) are is configured so that either no test line signal or just a signal at the first test line (T1) is detectable when βTP concentration in a sample applied to said device is lower than the βTP level indicative of a CSF leak; and said second test line (T2) is configured so that a signal is detectable at said second test line when βTP concentration in a sample applied to said device is greater than the βTP level indicative of a CSF leak.
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公开(公告)号：US20190391143A1
公开(公告)日：2019-12-26
申请号：US16434100
申请日：2019-06-06
IPC分类号： G01N33/558 , G01N33/543
摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
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公开(公告)号：US11867693B2
公开(公告)日：2024-01-09
申请号：US17530336
申请日：2021-11-18
发明人： Daniel Takashi Kamei , Maie A. St. John , Zachary D. Taylor , Daniel William Bradbury , Ashley Elizabeth Kita
IPC分类号： G01N33/543 , B01L3/00
CPC分类号： G01N33/54386 , B01L3/5023 , B01L2400/0406 , G01N2333/99
摘要： Devices and methods are provided for the detection of CSF in a biological sample. In certain embodiments the device is a lateral flow device comprising: a porous substrate; a sample addition zone disposed on or in said porous substrate; a detection zone disposed on or in said porous substrate where said detection zone comprises at least a first test line (T1) and a second test line (T2) each test line comprising binding moieties that bind a complex formed between beta-trace protein (βTP) and an indicator attached to a βTP binding molecule; wherein said porous substrate defines a flow path through which a sample applied to the sample addition zone flows under capillary action away from said sample addition zone into said detection zone; and wherein said first test line (T1) and said second test line (T2) are is configured so that either no test line signal or just a signal at the first test line (T1) is detectable when βTP concentration in a sample applied to said device is lower than the βTP level indicative of a CSF leak; and said second test line (T2) is configured so that a signal is detectable at said second test line when βTP concentration in a sample applied to said device is greater than the βTP level indicative of a CSF leak.
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公开(公告)号：US20240003875A1
公开(公告)日：2024-01-04
申请号：US18120329
申请日：2023-03-10
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/54388 , G01N33/54366 , G01N33/54386 , G01N33/558
摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.
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