公开(公告)号：US20190025277A1

公开(公告)日：2019-01-24

申请号：US16138658

申请日：2018-09-21

申请人： The Trustees of Boston University

发明人： Amit MELLER ,  Meni WANUNU

IPC分类号： G01N33/487 ,  C12Q1/6869

摘要： Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores −10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.

公开(公告)号：US20160033471A1

公开(公告)日：2016-02-04

申请号：US14775217

申请日：2014-03-14

申请人： THE TRUSTEES OF BOSTON UNIVERSITY

发明人： Amit MELLER ,  Nicolas Di FIORI

IPC分类号： G01N33/487 ,  G01N21/63 ,  G01N27/49

CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  G01N21/63 ,  G01N27/49 ,  G01N2201/06113 ,  C12Q2565/631

摘要： Optoelectronic control of solid-state nanopores and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. Methods for controlling the translocation speed of biopolymers through solid-state nanopores and methods for unblocking clogged pores by illuminating nanopores are described.

摘要翻译： 固态纳米孔的光电子控制及其应用。 纳米孔是非常敏感的单分子传感器。 电子束已被用于制备具有亚纳米分辨率的薄固态膜中的合成纳米孔。 描述了通过固态纳米孔控制生物聚合物的转运速度的方法以及通过照射纳米孔解除堵塞的孔的方法。

公开(公告)号：US20160103067A1

公开(公告)日：2016-04-14

申请号：US14815727

申请日：2015-07-31

申请人： The Trustees of Boston University

发明人： Amit MELLER ,  Meni WANUNU

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N2021/6439 ,  Y10T428/2982 ,  Y10T436/143333 ,  C12Q2521/543 ,  C12Q2565/631

摘要： Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores −19 nm diameter can be reproducibly ceased, and (2) self-assembly under voltage driven electrolyte flow, in which 5 nm nanopore may be coated. Applications of chemically modified nanopore are provided including, the detection of biopolymers such as DNA and RNA, immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.

摘要翻译： 固态纳米孔和纳米孔阵列的化学官能化及其应用。 纳米孔是非常敏感的单分子传感器。 最近，电子束已被用于制备具有亚纳米分辨率的薄固态膜中的合成纳米孔。 提供了一类新的化学修饰的纳米孔传感器，它们通过以下方式提供了纳米孔单层涂层的两种方法：（1）溶液的自组装，其中纳米孔-19nm直径可再现地停止，以及（2）自组装电压 驱动的电解质流动，其中可以涂覆5纳米的纳米孔。 提供化学修饰纳米孔的应用，包括检测生物聚合物如DNA和RNA，固定酶或其他蛋白质进行检测或产生化学梯度; 和局部pH感测。