公开(公告)号：US20210132054A1

公开(公告)日：2021-05-06

申请号：US16492128

申请日：2018-03-07

Applicant: THE TRUSTEES OF DARTMOUTH COLLEGE

Inventor： Amogha TADIMETY ,  John X.J. ZHANG

IPC: G01N33/543 ,  G01N21/552 ,  G01N33/553

Abstract: A method of making a plasmon-resonance biosensor includes conjugating precious metal nanorods with form factor at least 1.5 with a biological probe material. Microfluidic chambers of volume under 0.025 microliter are formed over a substrate, and an aqueous suspension of the conjugated nanorods is injected into the chambers. The nanorods are organized in rows and aligned in long dimension along the rows. The biosensor is configured to be read by obtaining an optical absorption spectrum upon exposure to the analyte. The biosensor includes precious metal nanorods organized in rows with long dimension approximately parallel to the rows. The nanorods are conjugated with biological probes capable of binding to an analyte, the probes may be an aptamer, an antibody, a protein-nucleic acid (PNA), a complimentary DNA, or an enzyme having a binding site.

公开(公告)号：US20240027439A1

公开(公告)日：2024-01-25

申请号：US18376380

申请日：2023-10-03

Applicant: THE TRUSTEES OF DARTMOUTH COLLEGE

Inventor： Amogha TADIMETY ,  John X.J. ZHANG

IPC: G01N33/543 ,  G01N21/552 ,  G01N33/553

CPC classification number: G01N33/54373 ,  G01N21/554 ,  G01N33/54346 ,  G01N33/553 ,  B82Y15/00

Abstract: A method of making a plasmon-resonance biosensor includes conjugating precious metal nanorods with form factor at least 1.5 with a biological probe material. Microfluidic chambers of volume under 0.025 microliter are formed over a substrate, and an aqueous suspension of the conjugated nanorods is injected into the chambers. The nanorods are organized in rows and aligned in long dimension along the rows. The biosensor is configured to be read by obtaining an optical absorption spectrum upon exposure to the analyte. The biosensor includes precious metal nanorods organized in rows with long dimension approximately parallel to the rows. The nanorods are conjugated with biological probes capable of binding to an analyte, the probes may be an aptamer, an antibody, a protein-nucleic acid (PNA), a complimentary DNA, or an enzyme having a binding site.