公开(公告)号：US20220389505A1

公开(公告)日：2022-12-08

申请号：US17812915

申请日：2022-07-15

申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Servic

发明人： Kapil Bharti ,  Janine Davis ,  Arvydas Maminishkis ,  Marc Ferrer-Alegre ,  Sheldon S. Miller

IPC分类号： C12Q1/6881 ,  C12N5/079 ,  G01N33/50 ,  G01N33/569

摘要： High efficiency methods for producing retinal pigment epithelial cells from induced pluripotent stem cells (iPSCs) are disclosed herein. The iPSCs are produced from somatic cells, including retinal pigment epithelial (RPE) cells, such as fetal RPE stem cells. In some embodiments, the iPSC include a tyrosinase promoter operably linked to a marker. Methods are disclosed for using the RPE cells, such as for treatment. Methods for screening for agents that affect RPE differentiation are also disclosed.

公开(公告)号：US20200178964A1

公开(公告)日：2020-06-11

申请号：US16348855

申请日：2017-11-08

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVIC

发明人： Vladimir R. Khristov ,  Steven T. Charles ,  Juan A. Amaral ,  Arvydas Maminishkis ,  Kapil Bharti

IPC分类号： A61B17/08 ,  A61B17/04 ,  A61B17/02

摘要： A surgical clamp for aligning the margins of incised or wounded tissue has jaws with parallel clamping faces, and a handle for manipulating the clamp to align the margins of the tissue. The jaws are in a normally closed position, however they can be opened by compressing the handle to open the jaws. Prongs project from the inferior surface of the jaws. The clamp is positioned in a desired position over the margins of a wound to be closed, the prongs engage the margins of the wound to be aligned, and the jaws are closed by releasing compressive force on the handle. As the jaws close the prongs help move the tissue into alignment. Suture guide slots through the jaws assist in the placement of precisely placed sutures across the incision. The disclosed surgical clamp is particularly suited for selectively closing and reopening surgical incisions, such as a sclerotomy incision in the eye. Methods are disclosed for using the clamp during intraocular and other surgical or minimally invasive procedures. In one example the clamp is used during implantation into the retina of a scaffold on which choroid and retinal pigment epithelium cells and retina grow in a three-dimensional matrix that mimics the native structure of the retina.