公开(公告)号：US10150946B2

公开(公告)日：2018-12-11

申请号：US14769724

申请日：2014-02-21

Applicant: The University of Sheffield

Inventor： Irene Canton ,  Steven Peter Armes ,  Nicholas John Warren ,  Harry Moore ,  Giuseppe Battaglia ,  Denis Cecchin

IPC: C12N5/00

Abstract: The present invention relates to a medium for storing, preserving, culturing, and/or differentiating stem cells. The medium employs a synthetic thermo-responsive copolymer, dispersed in an aqueous vehicle, which allows the medium to be readily “switched” between a non-gelatinous (fluid) form and a gelatinous form by simply adjusting temperature across the gelling temperature of the medium. As such, stem cells can be easily captured within a 3D gel matrix by simply mixing the stem cells with the non-gelatinous/fluid form of the medium and then gelling the medium by adjusting the temperature across the gelling temperature of the medium. The stem cells encapsulated within the gelatinous form of the medium may then be preserved for a significant time period before either using the stem cells directly within the medium (e.g. in therapy, or in culturing, differentiation, and such like), or after their extraction from the medium.

公开(公告)号：US20150376567A1

公开(公告)日：2015-12-31

申请号：US14769724

申请日：2014-02-21

Applicant: THE UNIVERSITY OF SHEFFIELD

Inventor： Irene Canton ,  Steven Peter Armes ,  Nicholas John Warren ,  Harry Moore ,  Guiseppe Battaglia ,  Denis Cecchin

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12N2533/40 ,  C12N2539/10

Abstract: The present invention relates to a medium for storing, preserving, culturing, and/or differentiating stem cells. The medium employs a synthetic thermo-responsive copolymer, dispersed in an aqueous vehicle, which allows the medium to be readily “switched” between a non-gelatinous (fluid) form and a gelatinous form by simply adjusting temperature across the gelling temperature of the medium. As such, stem cells can be easily captured within a 3D gel matrix by simply mixing the stem cells with the non-gelatinous/fluid form of the medium and then gelling the medium by adjusting the temperature across the gelling temperature of the medium. The stem cells encapsulated within the gelatinous form of the medium may then be preserved for a significant time period before either using the stem cells directly within the medium (e.g. in therapy, or in culturing, differentiation, and such like), or after their extraction from the medium.

Abstract translation: 本发明涉及用于储存，保存，培养和/或分化干细胞的培养基。 该介质使用分散在水性载体中的合成热响应性共聚物，其允许介质通过简单地调节介质的凝胶化温度之间的温度而容易地在非凝胶状（流体）形式和凝胶状之间“转换” 。 因此，通过简单地将干细胞与培养基的非凝胶状/流体形式混合，然后通过调节培养基的凝胶化温度的温度来胶化培养基，可以容易地在3D凝胶基质内捕获干细胞。 然后可以将包封在培养基中的干胶质细胞形式的干细胞保持一段相当长的时间，直到在培养基中直接使用干细胞（例如在治疗或培养，分化等）或在其提取后 从媒介。