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公开(公告)号:US20210341488A1
公开(公告)日:2021-11-04
申请号:US17241861
申请日:2021-04-27
发明人: Mao Fukuyama , Akihide Hibara , Ayuko Imai , Koji Shigemura , Manabu Tokeshi
摘要: A fluorescence polarization immunoassay that uses a fluorescent labeling substance in which a single domain antibody is labeled with a fluorescent dye. A fluorescent labeling substance obtained by labeling a single domain antibody, such as a VHH antibody or a vNAR antibody, having a binding ability to a target substance with a fluorescent dye is used. The fluorescence polarization immunoassay includes a binding step for binding the fluorescent labeling substance to a target substance; and a measuring step for measuring a change in the fluorescence polarization of the fluorescent labeling substance to which the target substance is bound.
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公开(公告)号:US09719927B2
公开(公告)日:2017-08-01
申请号:US15311311
申请日:2015-05-08
发明人: Akihide Hibara , Manabu Tokeshi , Osamu Wakao
IPC分类号: G01N21/64 , G02F1/13 , G01N33/542
CPC分类号: G01N21/6445 , G01J3/0224 , G01J4/00 , G01N21/64 , G01N21/6456 , G01N33/542 , G01N2201/0675 , G01N2201/0683 , G02F1/13
摘要: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1, and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.
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