公开(公告)号：US20210207085A1

公开(公告)日：2021-07-08

申请号：US16755490

申请日：2018-10-09

申请人： TOKYO INSTITUTE OF TECHNOLOGY ,  KANTO KAGAKU KABUSHIKI KAISHA ,  CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Shoen KUME ,  Nobuaki SHIRAKI ,  Hiroyuki YAMAGUCHI ,  Tomoaki INOUE ,  Toshito NAKAGAWA ,  Jumpei KIYOKAWA

IPC分类号： C12N5/071

摘要： The present invention provides, in order to prepare matured hepatocytes analogous in various points to primary hepatocytes, a method for preparing hepatocytes or cells that can be differentiated into hepatocytes from pluripotent stem cells, comprising the steps of: (1) culturing the pluripotent stem cells in a medium containing an activator of an activin receptor-like kinase-4,7; (2) culturing the cells obtained in the step (1) in a medium containing a bone morphogenetic factor and a fibroblast growth factor; (3) culturing the cells obtained in the step (2) in a medium containing an activator of a hepatocyte growth factor receptor and an activator of an oncostatin M receptor; and (4) culturing the cells obtained in the step (3) to obtain hepatocytes or cells that can be differentiated into hepatocytes, wherein in at least one of the steps (2), (3) and (4), cells are cultured on a high-density collagen gel membrane.

公开(公告)号：US20210190764A1

公开(公告)日：2021-06-24

申请号：US16756415

申请日：2018-10-19

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： Yuki NOGUCHI ,  Takeru NAMBU ,  Kazuhisa OZEKI ,  Noriaki OHMINATO ,  Toshito NAKAGAWA ,  Sotaro NAOI

IPC分类号： G01N33/50

摘要： The present invention provides a method for measuring a cellular uptake amount of a molecule, comprising (i) adding the molecule to an organ-derived cell population to perform incubation, (ii) sorting the organ-derived cell population based on the expression levels of CD31 and CD45, and (iii) after steps (i) and (ii), measuring the amount of the molecule incorporated into the cell population sorted in the step (ii), wherein the molecule is incorporated into cells via a cell surface receptor.