公开(公告)号：US20240026367A1

公开(公告)日：2024-01-25

申请号：US18467967

申请日：2023-09-15

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/82 ,  C12N15/63 ,  C12N9/16 ,  C12N15/11 ,  C12N15/10 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22

CPC分类号： C12N15/52 ,  C12N15/8216 ,  C12N15/63 ,  C12N9/16 ,  C12N15/111 ,  C12N15/102 ,  C12N15/907 ,  C12N15/85 ,  C12N9/22 ,  C12Y301/21 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531

摘要： The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides compositions for inducing targeted disruption of endogenous genes in eukaryotic cells. The composition may comprise a Cas9/guide RNA complex with a Cas9 protein to which a NLS is linked, and a guide RNA having a crRNA and a tracrRNA. The crRNA may comprise i) a portion to be hybridized with a portion of the tracrRNA, and ii) a portion complementary to a target DNA of the endogenous genes. In some embodiments, the Cas/guide RNA complex may be formed in vitro before being introduced into the eukaryotic cell.

公开(公告)号：US20230374524A1

公开(公告)日：2023-11-23

申请号：US18313946

申请日：2023-05-08

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/82 ,  C12N15/63 ,  C12N9/16 ,  C12N15/11 ,  C12N15/10 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22

CPC分类号： C12N15/52 ,  C12N15/8216 ,  C12N15/63 ,  C12N9/16 ,  C12N15/111 ,  C12N15/102 ,  C12N15/907 ,  C12N15/85 ,  C12N9/22 ,  C12Y301/21 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20150284727A1

公开(公告)日：2015-10-08

申请号：US14438098

申请日：2013-10-23

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim ,  Jong Min Kim ,  Seokjoong Kim

IPC分类号： C12N15/52 ,  C12N9/16

CPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531 ,  C12Y301/21

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

摘要翻译： 本发明涉及真核细胞或生物体中靶向的基因组编辑。 更具体地说，本发明涉及用于在真核细胞或生物体中切割靶DNA的组合物，其包含对靶DNA和Cas蛋白质编码核酸或Cas蛋白质特异性的引导RNA及其用途。

公开(公告)号：US20240240192A9

公开(公告)日：2024-07-18

申请号：US18467952

申请日：2023-09-15

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90

CPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/907 ,  C12Y301/21 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531

摘要： The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for Cas9/RNA complexes that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. The Cas9/RNA complex may comprise a recombinant Cas9 protein including a nuclear localization signal (NLS) and a guide RNA including a crRNA and a tracrRNA. The Cas9/RNA complex may be a combination of the recombinant Cas9 protein and the guide RNA. The guide RNA may be transcribed in vitro or synthesized chemically. The target endogenous nucleic acid sequence may include a portion complementary to the crRNA of the guide RNA.

公开(公告)号：US20240052356A1

公开(公告)日：2024-02-15

申请号：US18467952

申请日：2023-09-15

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/82 ,  C12N15/63 ,  C12N9/16 ,  C12N15/11 ,  C12N15/10 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22

CPC分类号： C12N15/52 ,  C12N15/8216 ,  C12N15/63 ,  C12N9/16 ,  C12N15/111 ,  C12N15/102 ,  C12N15/907 ,  C12N15/85 ,  C12N9/22 ,  C12Y301/21 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531

摘要： The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for Cas9/RNA complexes that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. The Cas9/RNA complex may comprise a recombinant Cas9 protein including a nuclear localization signal (NLS) and a guide RNA including a crRNA and a tracrRNA. The Cas9/RNA complex may be a combination of the recombinant Cas9 protein and the guide RNA. The guide RNA may be transcribed in vitro or synthesized chemically. The target endogenous nucleic acid sequence may include a portion complementary to the crRNA of the guide RNA.

公开(公告)号：US20230374525A1

公开(公告)日：2023-11-23

申请号：US18314050

申请日：2023-05-08

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/90 ,  C12N9/16 ,  C12N15/82 ,  C12N15/85 ,  C12N15/63 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11

CPC分类号： C12N15/52 ,  C12N15/907 ,  C12N9/16 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/63 ,  C12Y301/21 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N2310/531 ,  C12N2310/10 ,  C12N2310/20

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20150344912A1

公开(公告)日：2015-12-03

申请号：US14685510

申请日：2015-04-13

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/90

CPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/531 ,  C12Y301/21

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20210047648A1

公开(公告)日：2021-02-18

申请号：US17004355

申请日：2020-08-27

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US20210047647A1

公开(公告)日：2021-02-18

申请号：US17004338

申请日：2020-08-27

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/52 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.

公开(公告)号：US10851380B2

公开(公告)日：2020-12-01

申请号：US14685568

申请日：2015-04-13

申请人： TOOLGEN INCORPORATED

发明人： Jin-Soo Kim ,  Seung Woo Cho ,  Sojung Kim

IPC分类号： C12N15/85 ,  C12N15/52 ,  C12N15/90 ,  C12N9/22 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/11 ,  C12N9/16

摘要： The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.