公开(公告)号：US20170191123A1

公开(公告)日：2017-07-06

申请号：US15314288

申请日：2015-05-28

Applicant: TOOLGEN INCORPORATED ,  INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  So Jung Kim ,  Seung Hwan Lee ,  Seok Joong Kim

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/68 ,  C12Q2521/301 ,  G01N33/53 ,  G01N33/574

Abstract: The present invention relates to a method for analyzing a genotype using a target-specific nuclease and, specifically, to a method for diagnosing cancer or analyzing a genotype by removing wild type DNA or particular genotype DNA using a target-specific nuclease or a variant thereof to amplify or concentrate only a small amount of DNA which has a difference in variation, such as a mutation, or genotype, and to a method for separating target DNA sesusing a target-specific nuclease or a variant thereof. Such methods are novel paradigm methods contrary to existing simple target-specific nucleases for post-PCR recognition of normal genotype and carcinogenic genotype, and can be favorably used in the early diagnosis of cancer or analysis of similar genotypes.