公开(公告)号：US20230117082A1

公开(公告)日：2023-04-20

申请号：US18067264

申请日：2022-12-16

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Akihiro HOSHINO ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yosuke HORIUCHI

IPC: B01L3/00 ,  G01N21/64

Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

公开(公告)号：US20220340953A1

公开(公告)日：2022-10-27

申请号：US17806099

申请日：2022-06-09

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: C12Q1/682

Abstract: A detection method including bringing liquid including a target nucleic acid into contact with a well array having wells such that at most one molecule of the target nucleic acid is included per well, sealing the well such that the target nucleic acid remains in the well, amplifying, in the well, a signal derived from the target nucleic acid, detecting the signal emitted from the well, and detecting whether the target nucleic acid includes at least one of a first nucleic acid, a second nucleic acid, and a double-stranded nucleic acid resulting from complementary binding of the first and second nucleic acids. The signal includes at least one of a first signal emitted by binding of a first specific binding substance to the first nucleic acid, and a second signal different from the first signal and emitted by binding of a second specific binding substance to the second nucleic acid.

公开(公告)号：US20220088598A1

公开(公告)日：2022-03-24

申请号：US17537650

申请日：2021-11-30

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: B01L3/00

Abstract: A method of replacing liquids including introducing a second liquid into a fluidic device which includes a substrate having wells formed on a surface thereof, the wells including openings which accommodate a first liquid including a surfactant and are sealed with a first sealing solution applied on the surface of the substrate, where the introducing includes supplying the second liquid to the surface such that the first sealing solution is replaced with the second liquid, and that the second liquid is introduced into the wells.