公开(公告)号：US20230201826A1

公开(公告)日：2023-06-29

申请号：US18116411

申请日：2023-03-02

Applicant: TOPPAN Inc.

Inventor： Keisuke GOTO ,  Yoichi MAKINO ,  Shinichi WADA ,  Yuichiro SHINOHARA

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L2300/0829 ,  B01L2200/0689 ,  B01L2300/041

Abstract: A fluidic device including a substrate having a well array that includes regularly arranged wells that have a same shape and are open to a surface of the substrate, and a cover member facing the well array. The well array and the cover member are positioned to have a space therebetween, which forms a flow path through which a fluid flows, and the wells including a well A and a well B closest to the well A satisfy formula (1): 0.8≤Da/Dab

公开(公告)号：US20220333175A1

公开(公告)日：2022-10-20

申请号：US17809916

申请日：2022-06-30

Applicant: TOPPAN Inc.

Inventor： Masahiro MATSUKAWA ,  Yoichi MAKINO ,  Akihiro HOSHINO

IPC: C12Q1/6844 ,  G01N33/68 ,  C12Q1/68 ,  G01N21/00 ,  C12M1/00 ,  G01N37/00 ,  C12N15/09 ,  G01N21/64 ,  G01N33/50 ,  B01L3/00 ,  C12M1/34

Abstract: A reaction container includes a transparent base including a resin material and having recessed portions formed in one or more regions of the transparent base such that the recessed portions are recessed from a surface of the transparent base, and a cover member including a thermoplastic resin material and positioned on the transparent base such that the cover member forms a flow path including a gap extending over the recessed portions between the cover member and the surface of the transparent base and has a spacer portion welded by laser irradiation to the transparent base outside the one or more regions of the transparent base. The cover member absorbs infrared light and transmits light having a wavelength within a range of visible light such that the cover member has light transmittance in the range of from 480 nm to 570 nm.

公开(公告)号：US20220106648A1

公开(公告)日：2022-04-07

申请号：US17531983

申请日：2021-11-22

Applicant: TOPPAN Inc.

Inventor： Takumi HIRASE ,  Yoichi MAKINO

IPC: C12Q1/70 ,  G01N33/569

Abstract: A method for detecting a target molecule of a structure body, including contacting a dispersion liquid including a structure body with a well array including wells such that the structure body is introduced into the wells, bringing a sealing solution into contact with the well array such that the structure body is sealed in at least one of the wells, extracting a content of the structure body in the well, detecting a surface target molecule present on a surface of the structure body in the well, and detecting an internal target molecule present inside the structure body in the well.

公开(公告)号：US20230251197A1

公开(公告)日：2023-08-10

申请号：US18303700

申请日：2023-04-20

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Keisuke GOTO

IPC: G01N21/64 ,  C12Q1/68 ,  G01N33/487 ,  B01L3/00 ,  G01N21/05 ,  G01N35/08 ,  C12Q1/25

CPC classification number: G01N21/645 ,  C12Q1/68 ,  G01N33/487 ,  B01L3/502715 ,  G01N21/05 ,  G01N35/08 ,  C12Q1/25 ,  G01N21/6428 ,  B01L2300/0877 ,  G01N2021/6482 ,  B01L2300/165 ,  B01L2300/0829 ,  B01L2300/0887 ,  G01N2021/058

Abstract: A microfluidic device includes a substrate having an electromagnetic wave transmission property, a micropore array layer formed on the substrate and having microwells such that the microwells is configured to receive a target of analysis, and a light absorption layer formed over the microwells of the micropore array layer such that the light absorption layer absorbs an electromagnetic wave.

公开(公告)号：US20230113730A1

公开(公告)日：2023-04-13

申请号：US17913679

申请日：2021-02-18

Applicant: TOPPAN INC. ,  KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

Inventor： Yoichi MAKINO ,  Yoshizumi ISHINO ,  Sonoko ISHINO

IPC: C12Q1/6823

Abstract: The present invention relates to a method for detecting a target nucleic acid, the method including cleaving a first flap of a first cleavage structure formed by a target nucleic acid, a first nucleic acid, and a second nucleic acid; cleaving a second flap of a second cleavage structure formed by a third nucleic acid, the cleaved first flap, and a fourth nucleic acid; and detecting the presence of the target nucleic acid by detecting the cleaved second flap, wherein cleaving the first flap and cleaving the second flap are carried out by cleaving the first flap and the second flap with a flap endonuclease, and the flap endonuclease has an amino acid sequence having a sequence identity of 96% or higher with an amino acid sequence of a flap endonuclease of Thermococcus kodakarensis strain KOD1, or the like.

公开(公告)号：US20230126262A1

公开(公告)日：2023-04-27

申请号：US17912220

申请日：2021-02-18

Applicant: TOPPAN INC. ,  KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

Inventor： Yoichi MAKINO ,  Yoshizumi ISHINO ,  Sonoko ISHINO

IPC: C12Q1/6823

Abstract: The present invention relates to a method for detecting a target nucleic acid, the method including cleaving a first flap of a first cleavage structure formed by a target nucleic acid, a first nucleic acid, and a second nucleic acid; cleaving a second flap of a second cleavage structure formed by a third nucleic acid, the cleaved first flap, and a fourth nucleic acid; and detecting the presence of the target nucleic acid by detecting the cleaved second flap, wherein cleaving the first flap and cleaving the second flap are carried out by cleaving the first flap and the second flap with a flap endonuclease, and the flap endonuclease has an amino acid sequence having a sequence identity of 65% or higher with an amino acid sequence of a flap endonuclease of a microbe selected from the group consisting of microbes belonging to the Order Thermococcales and microbes belonging to the Order Methanobacteriales.

公开(公告)号：US20230117082A1

公开(公告)日：2023-04-20

申请号：US18067264

申请日：2022-12-16

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Akihiro HOSHINO ,  Tomoko KUNITOMI ,  Keisuke GOTO ,  Yosuke HORIUCHI

IPC: B01L3/00 ,  G01N21/64

Abstract: An analysis apparatus including a stage, an analysis device placed on the stage and including receiving sections which accommodate a sample and a reagent for biochemical reaction, and are communicated with one another through a flow path having an inlet and an outlet, a liquid introduction section which is connected to the inlet and supplies into the flow path the sample, the reagent, and an sealing liquid for sealing each of the receiving sections, and a waste liquid storage section which is connected to the outlet and stores as waste liquid an excess of the sample and the reagent and a part of the sealing liquid supplied to the flow path, an optical system which includes an objective lens, emits excitation light to the receiving sections and allows observation of fluorescence generated in the receiving sections by the excitation light, and a control unit that controls such that the sealing liquid and the excess of the sample and the reagent form an interface in the waste liquid storage section, and that the interface is formed at a distance not less than a fluorescence-obtainable distance from a bottom of the receiving sections.

公开(公告)号：US20220340953A1

公开(公告)日：2022-10-27

申请号：US17806099

申请日：2022-06-09

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: C12Q1/682

Abstract: A detection method including bringing liquid including a target nucleic acid into contact with a well array having wells such that at most one molecule of the target nucleic acid is included per well, sealing the well such that the target nucleic acid remains in the well, amplifying, in the well, a signal derived from the target nucleic acid, detecting the signal emitted from the well, and detecting whether the target nucleic acid includes at least one of a first nucleic acid, a second nucleic acid, and a double-stranded nucleic acid resulting from complementary binding of the first and second nucleic acids. The signal includes at least one of a first signal emitted by binding of a first specific binding substance to the first nucleic acid, and a second signal different from the first signal and emitted by binding of a second specific binding substance to the second nucleic acid.

公开(公告)号：US20220088598A1

公开(公告)日：2022-03-24

申请号：US17537650

申请日：2021-11-30

Applicant: TOPPAN Inc.

Inventor： Yoichi MAKINO ,  Yosuke HORIUCHI

IPC: B01L3/00

Abstract: A method of replacing liquids including introducing a second liquid into a fluidic device which includes a substrate having wells formed on a surface thereof, the wells including openings which accommodate a first liquid including a surfactant and are sealed with a first sealing solution applied on the surface of the substrate, where the introducing includes supplying the second liquid to the surface such that the first sealing solution is replaced with the second liquid, and that the second liquid is introduced into the wells.