公开(公告)号：US20240174982A1

公开(公告)日：2024-05-30

申请号：US18437415

申请日：2024-02-09

Applicant: TOPPAN Inc.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO ,  Yuma YOKOKAWA

IPC: C12N5/077 ,  C12N5/09

CPC classification number: C12N5/0669 ,  C12N5/0693 ,  C12N2501/90 ,  C12N2501/998 ,  C12N2513/00 ,  C12N2533/56 ,  C12N2533/76

Abstract: A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cellular tissues.