公开(公告)号：US20230287356A1

公开(公告)日：2023-09-14

申请号：US18319691

申请日：2023-05-18

Applicant: TOPPAN Inc.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/56 ,  C12N2533/54 ,  C12N2501/91

Abstract: A method of producing a three-dimensional cell tissue include obtaining a mixture including cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, gelling the mixture such that a gel composition including the cells, cationic substance, extracellular matrix component and polyelectrolyte is obtained; and incubating the gel composition such that a three-dimensional cell tissue is obtained.

公开(公告)号：US20230203452A1

公开(公告)日：2023-06-29

申请号：US18171832

申请日：2023-02-21

Applicant: TOPPAN Inc.

Inventor： Kei TSUKAMOTO ,  Shiro KITANO ,  Tomoko KUNITOMI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12N2502/28 ,  C12N2502/1323 ,  C12N2513/00 ,  C12N2533/90

Abstract: A method of producing a conditioned medium for culturing a patient-derived cancer cell, including culturing, in a first medium for 24 hours or longer, a three-dimensional cell tissue including an extracellular matrix component, a polymer electrolyte, and a cell cluster including a fibroblast, and collecting the first medium in which the three-dimensional cell tissue has been cultured as the conditioned medium.

公开(公告)号：US20240174982A1

公开(公告)日：2024-05-30

申请号：US18437415

申请日：2024-02-09

Applicant: TOPPAN Inc.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO ,  Yuma YOKOKAWA

IPC: C12N5/077 ,  C12N5/09

CPC classification number: C12N5/0669 ,  C12N5/0693 ,  C12N2501/90 ,  C12N2501/998 ,  C12N2513/00 ,  C12N2533/56 ,  C12N2533/76

Abstract: A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cellular tissues.

公开(公告)号：US20230323310A1

公开(公告)日：2023-10-12

申请号：US18043507

申请日：2021-09-03

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Tomoko KUNITOMI ,  Shiro KITANO ,  Kei TSUKAMOTO ,  Michiya MATSUSAKI

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2501/91

Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.