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    METHOD FOR PREPARING BONE MARROW CELL AGGREGATE
    4.
    发明申请
    METHOD FOR PREPARING BONE MARROW CELL AGGREGATE 审中-公开

    公开(公告)号：US20170321191A1

    公开(公告)日：2017-11-09

    申请号：US15661654

    申请日：2017-07-27

    申请人： PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY

    发明人： Nobuhiko KOJIMA

    IPC分类号： C12N5/077

    CPC分类号： C12N5/0669 ,  A61L27/00 ,  C12M3/00 ,  C12N5/00 ,  C12N2513/00 ,  C12N2533/70 ,  C12N2533/78 ,  C12N2539/00

    摘要： The present invention provides a technique which enables organization of bone marrow cells by a simple method in a short period of time.A method for preparing a bone marrow cell aggregate, comprising adding a liquid containing a bone marrow cell population to a medium containing a swellable material and culturing the bone marrow cell population in the presence of the swellable material. A method for reassembling a bone marrow tissue, comprising adding a liquid containing a bone marrow cell population to a medium containing a swellable material and culturing the bone marrow cell population in the presence of the swellable material.According to common knowledge in the art, it has been considered difficult to reorganize once disintegrated bone marrow tissue without changing the cell composition (that is, without adding any adherent cell or extracellular matrix which will work as a “connecting material (binder)”). Indeed, it was impossible to aggregate bone marrow cells by conventional methods. As a result of its achievement, the present invention changes such conventional thought and results and provides a major breakthrough technique pertaining to 3D culture of bone marrow cells. It has also been confirmed that culture of a bone marrow-like tissue reassembled by the method of the present could be continued up to day 14 in the MC medium.

    Sophisticated Implant Material
    10.
    发明申请
    Sophisticated Implant Material 审中-公开
    复杂的种植体材料

    公开(公告)号：US20160193387A1

    公开(公告)日：2016-07-07

    申请号：US14655990

    申请日：2013-12-26

    申请人： QUARRYMEN CORPORATION

    发明人： Minoru Ueda

    IPC分类号： A61L27/38 ,  A61L27/24 ,  C12N5/077 ,  A61L27/02 ,  A61L27/12 ,  A61L27/22 ,  A61L27/06

    CPC分类号： A61L27/3834 ,  A61L27/025 ,  A61L27/04 ,  A61L27/06 ,  A61L27/12 ,  A61L27/227 ,  A61L27/24 ,  A61L27/3895 ,  A61L27/54 ,  A61L2300/414 ,  A61L2300/64 ,  A61L2430/02 ,  C12N5/0664 ,  C12N5/0669 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/71 ,  C12N2501/998

    摘要： The purpose of the present invention is to provide: a method for preparing a cell product for use in the production of an implant, which can improve the surface properties of a material by a simple technique and can integrate a bone with a metallic implant material satisfactorily within a given period of time; a product produced by the method; and a sophisticated implant produced using the product. A cell product containing Col-I, OCN, OPN, BSP, fibronectin and VEGF or another growth factor which are produced by a bone marrow stromal cell or an immortalized milk teeth stem cell is prepared from the bone marrow stromal cell or the immortalized milk teeth stem cell, and a sophisticated implant having the growth factor immobilized on the surface thereof is provided using the resultant cell product and a mineralizing solution.

    摘要翻译： 本发明的目的是提供：一种用于制备用于生产植入物的细胞产品的方法，其可以通过简单的技术改善材料的表面性质，并且可以令人满意地将骨骼与金属植入材料整合 在给定的时间内; 通过该方法生产的产品; 以及使用该产品生产的复杂植入物。 从骨髓基质细胞或永生化的乳牙齿制备含有由骨髓基质细胞或永生化的乳牙干细胞产生的Col-1，OCN，OPN，BSP，纤连蛋白和VEGF或另一种生长因子的细胞产物 使用所得的细胞产物和矿化溶液提供干细胞和具有固定在其表面上的生长因子的复杂植入物。