公开(公告)号：US20140073033A1

公开(公告)日：2014-03-13

申请号：US14077621

申请日：2013-11-12

申请人： TRANSGENE S.A.

发明人： Claude SENE ,  Sylvie CAMPOURCY ,  Yves CORDIER

IPC分类号： C12N7/00

CPC分类号： C12N7/025 ,  A61K35/768 ,  C12N7/00 ,  C12N7/02 ,  C12N15/86 ,  C12N2710/24152 ,  C12N2710/24251 ,  C12P21/00 ,  Y02A50/388 ,  Y02A50/39

摘要： The present invention relates to a method for producing and purifying a wild type, an attenuated and/or a recombinant Orthopoxvirus. The present invention relates to a purified wild type, attenuated and/or recombinant Orthopoxvirus obtained by the method of the invention and to a pharmaceutical composition, preferably a vaccine, comprising said purified Orthopoxvirus for the treatment and/or the prevention a cancer, an infectious disease and/or an autoimmune disorder, and uses thereof The present invention also relates to the use of an immortalized avian cell line obtained from an avian cell belonging to the Anatidae family, in particular Cairina moschata immortalized avian cell lines comprising a nucleic acid sequence coding a telomerase reverse transcriptase (TERT) and optionally an E1A nucleic acid sequence, for the production of a wild type, attenuated and/or recombinant Orthopoxvirus according to the process of the invention

摘要翻译： 本发明涉及野生型，减毒型和/或重组型直痘病毒的生产和纯化方法。 本发明涉及通过本发明方法获得的纯化的野生型，减毒和/或重组的直痘病毒，以及包含用于治疗和/或预防癌症的感染性的包含所述纯化的直痘病毒的药物组合物，优选疫苗 疾病和/或自身免疫性疾病及其用途本发明还涉及从属于id科家族的鸟类细胞获得的永生化鸟类细胞系的用途，特别是Cairina moschata永生化鸟类细胞系，其包含编码 根据本发明的方法生产野生型，减毒和/或重组的直链痘病毒的端粒酶逆转录酶（TERT）和任选的E1A核酸序列