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公开(公告)号:US09200306B2
公开(公告)日:2015-12-01
申请号:US13836817
申请日:2013-03-15
Applicant: TSINGHUA UNIVERSITY
Inventor: Zhanglin Lin , Wanghui Xu , Lei Xing , Lingli Zeng , Bihong Zhou , Qing Zhao , Wei Wu
IPC: C12P21/06 , C12N1/00 , C07K14/00 , C07K14/575 , C07K14/605
CPC classification number: C12P21/06 , C07K14/00 , C07K14/57581 , C07K14/605 , C07K2319/01 , C07K2319/50 , C07K2319/70 , C12N1/00
Abstract: The present invention relates to a method for production and purification of polypeptides. In particular, the present invention relates to a fusion protein comprising a solubility-enhancing peptide tag moiety, a self-aggregating peptide moiety and a moiety of target peptide and to a method for production and purification of target peptides through expressing said fusion protein.
Abstract translation: 本发明涉及多肽的制备和纯化方法。 特别地,本发明涉及包含溶解度增强肽标签部分,自聚集肽部分和靶肽部分的融合蛋白,以及通过表达所述融合蛋白产生和纯化靶肽的方法。