公开(公告)号：US09200306B2

公开(公告)日：2015-12-01

申请号：US13836817

申请日：2013-03-15

Applicant: TSINGHUA UNIVERSITY

Inventor： Zhanglin Lin ,  Wanghui Xu ,  Lei Xing ,  Lingli Zeng ,  Bihong Zhou ,  Qing Zhao ,  Wei Wu

IPC: C12P21/06 ,  C12N1/00 ,  C07K14/00 ,  C07K14/575 ,  C07K14/605

CPC classification number: C12P21/06 ,  C07K14/00 ,  C07K14/57581 ,  C07K14/605 ,  C07K2319/01 ,  C07K2319/50 ,  C07K2319/70 ,  C12N1/00

Abstract: The present invention relates to a method for production and purification of polypeptides. In particular, the present invention relates to a fusion protein comprising a solubility-enhancing peptide tag moiety, a self-aggregating peptide moiety and a moiety of target peptide and to a method for production and purification of target peptides through expressing said fusion protein.

Abstract translation: 本发明涉及多肽的制备和纯化方法。 特别地，本发明涉及包含溶解度增强肽标签部分，自聚集肽部分和靶肽部分的融合蛋白，以及通过表达所述融合蛋白产生和纯化靶肽的方法。

公开(公告)号：US11136360B2

公开(公告)日：2021-10-05

申请号：US16720456

申请日：2019-12-19

Applicant: Tsinghua University ,  South China University of Technology

Inventor： Zhanglin Lin ,  Tingting Wang ,  Qing Zhao ,  Xu Wang ,  Bihong Zhou ,  Lei Xing

IPC: C07K14/35 ,  C07K1/14

Abstract: The present disclosure describes a Mtu ΔI-CM intein variant containing one or more mutations or a biologically active fragment thereof, and a method for producing and purifying a molecule of interest using the intein variant. Further described are isolated fusion proteins comprising the intein variant and a tag and a molecule of interest. Also described are expression systems for expressing the intein variant as well as polypeptide screening methods employing the intein variant.