公开(公告)号：US20100216232A1

公开(公告)日：2010-08-26

申请号：US12506572

申请日：2009-07-21

申请人： TSUI-JUNG CHANG ,  CHIH-HSIEN CHANG ,  YA-JEN CHANG ,  TE-WEI LEE ,  TIN-YUN HO ,  CHIEN-YUN HSIANG

发明人： TSUI-JUNG CHANG ,  CHIH-HSIEN CHANG ,  YA-JEN CHANG ,  TE-WEI LEE ,  TIN-YUN HO ,  CHIEN-YUN HSIANG

IPC分类号： C12N5/10 ,  C12N5/071

CPC分类号： G01N33/5008

摘要： A mammal dedicated cell line is provided, which is a HepG2 hepatocellular carcinoma cell line (HepG2/NF-kB/Luc/sr39tk)1_18 obtained by co-transformation with NF-kB/Luc and NF-kB/sr39tk. Firstly, a successfully transformed pNF-kB/Luc HepG2 cell is obtained. Then, a dedicated cell line sensitive to TPA and MTX is generated by experimental screening Next, a plasmid construct carrying pNF-kB/sr39tk genome is introduced into the dedicated cell line by means of Superfect protocol. Finally, a HepG2 cell line co-expressing NF-kB/Luc and NF-kB/sr39tk is screened with G418 and ZEOCIN, and transformation result is confirmed by luminescence and radio activity. The (HepG2/NF-kB/Luc/sr39tk)1_18 obtained is suitable to screen drug for treating liver cancer and examine these cells by bioluminescence imaging and nuclear medicine imaging.

摘要翻译： 提供哺乳动物专用细胞系，其是通过与NF-kB / Luc和NF-kB / sr39tk共转化获得的HepG2肝细胞癌细胞系（HepG2 / NF-kB / Luc / sr39tk）1-18。 首先，获得成功转化的pNF-kB / Luc HepG2细胞。 然后，通过实验筛选产生对TPA和MTX敏感的专用细胞系。接下来，通过Superfect方案将携带pNF-kB / sr39tk基因组的质粒构建体导入专用细胞系。 最后，用G418和ZEOCIN筛选共表达NF-kB / Luc和NF-kB / sr39tk的HepG2细胞株，通过发光和放射活性证实转化结果。 获得的（HepG2 / NF-kB / Luc / sr39tk）1_18适用于筛选药物治疗肝癌，并通过生物发光成像和核医学成像检查这些细胞。

公开(公告)号：US20110124557A1

公开(公告)日：2011-05-26

申请号：US12949196

申请日：2010-11-18

申请人： TIN-YUN HO ,  CHIEN-YUN HSIANG

发明人： TIN-YUN HO ,  CHIEN-YUN HSIANG

IPC分类号： A61K38/10 ,  A61K38/16 ,  A61P3/10 ,  A61P3/08

CPC分类号： A61K38/04 ,  A61K38/16

摘要： A blood sugar-modulating polypeptide, a pharmaceutical composition comprising the polypeptide, and a method for modulating blood sugar in a mammal comprising the administration of the polypeptide are provided. The polypeptide has a following amino acid sequence or a homologous amino acid sequence derived from the substitution, deletion, and/or addition of one or more amino acids therein: RVRVWVTERGIVARPPTIG.(SEQ ID NO. 11)

摘要翻译： 提供了血糖调节多肽，包含该多肽的药物组合物，以及一种调节哺乳动物血糖的方法，包括多肽的给药。 多肽具有以下氨基酸序列或衍生自其中一个或多个氨基酸的取代，缺失和/或添加的同源氨基酸序列：RVRVWVTERGIVARPPTIG。 （SEQ ID NO.11）