公开(公告)号：US5804394A

公开(公告)日：1998-09-08

申请号：US138076

申请日：1993-10-20

申请人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

发明人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

IPC分类号： C07K16/40 ,  G01N33/573 ,  A61K39/395

CPC分类号： C07K16/40 ,  Y10S435/81 ,  Y10S436/811

摘要： The present invention provides such a measuring method of the CK isoform and a reagent therefor. Accordingly, the present invention provides a reagent for measuring creatine kinase (CK) activity comprising an antibody, wherein said antibody inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit. The present invention also provides a method for measuring CK activity comprising determining an inhibition of CK of body fluids using a reagent for measuring CK activity, wherein said reagent comprises an antibody which inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.

公开(公告)号：US5968756A

公开(公告)日：1999-10-19

申请号：US916262

申请日：1997-08-22

申请人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

发明人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

IPC分类号： C07K16/40 ,  G01N33/573 ,  A61K39/395

CPC分类号： C07K16/40 ,  Y10S435/81 ,  Y10S436/811

摘要： The present invention provides such a measuring method of the CK isoform and a reagent therefor. Accordingly, the present invention provides a reagent for measuring creatine kinase (CK) activity comprising an antibody, wherein said antibody inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.The present invention also provides a method for measuring CK activity comprising determining an inhibition of CK of body fluids using a reagent for measuring CK activity, wherein said reagent comprises an antibody which inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.

摘要翻译： 本发明提供了CK同种型及其试剂的测定方法。 因此，本发明提供了一种测定肌酸激酶（CK）活性的试剂，其含有抗体，其中所述抗体抑制CK-MT亚基，但不抑制CK-MS亚基。 本发明还提供了一种测定CK活性的方法，包括使用测定CK活性的试剂测定体液CK的抑制，其中所述试剂含有抑制CK-MT亚基但不抑制CK-MS亚基的​​抗体。

公开(公告)号：US5166049A

公开(公告)日：1992-11-24

申请号：US610679

申请日：1990-11-08

申请人： Akihiro Shinzaki ,  Mihoko Era ,  Naoko Kenmotsu ,  Tadao Suzuki

发明人： Akihiro Shinzaki ,  Mihoko Era ,  Naoko Kenmotsu ,  Tadao Suzuki

IPC分类号： C12Q1/26

CPC分类号： C12Q1/26 ,  Y10S435/81

摘要： A method for measuring diaphorase activity comprising mixing a sample comprising diaphorase with nitro blue tetrazolium, EDTA or a salt thereof, at least one of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate, and a surface active agent, to form an assay solution; and measuring an increase in absorbance, due to the formation of diformazan, in the assay solution and reagents used in a method for measuring diaphorase activity comprising a first solution, a second solution, EDTA, and a surface active agent, wherein the first solution comprises at least one of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate and the second solution comprises nitro blue tetrazolium.