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    Reagent for measuring creatine kinase activity and measuring method thereof
    2.
    发明授权
    Reagent for measuring creatine kinase activity and measuring method thereof 失效
    用于测量肌酸激酶活性的试剂及其测定方法

    公开(公告)号：US5968756A

    公开(公告)日：1999-10-19

    申请号：US916262

    申请日：1997-08-22

    申请人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

    发明人： Tadao Suzuki ,  Tomoko Kamei ,  Mihoko Era ,  Hiroyuki Tsubota

    IPC分类号： C07K16/40 ,  G01N33/573 ,  A61K39/395

    CPC分类号： C07K16/40 ,  Y10S435/81 ,  Y10S436/811

    摘要： The present invention provides such a measuring method of the CK isoform and a reagent therefor. Accordingly, the present invention provides a reagent for measuring creatine kinase (CK) activity comprising an antibody, wherein said antibody inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.The present invention also provides a method for measuring CK activity comprising determining an inhibition of CK of body fluids using a reagent for measuring CK activity, wherein said reagent comprises an antibody which inhibits CK-M.sub.T subunit, but does not inhibit CK-M.sub.S subunit.

    摘要翻译： 本发明提供了CK同种型及其试剂的测定方法。 因此，本发明提供了一种测定肌酸激酶（CK）活性的试剂，其含有抗体，其中所述抗体抑制CK-MT亚基，但不抑制CK-MS亚基。 本发明还提供了一种测定CK活性的方法，包括使用测定CK活性的试剂测定体液CK的抑制，其中所述试剂含有抑制CK-MT亚基但不抑制CK-MS亚基的​​抗体。

    Method for producing a thermostable lipoprotein lipase from streptomyces
    3.
    发明授权
    Method for producing a thermostable lipoprotein lipase from streptomyces 失效
    从链霉菌制备热稳定性脂蛋白脂肪酶的方法

    公开(公告)号：US5232846A

    公开(公告)日：1993-08-03

    申请号：US929744

    申请日：1992-09-03

    申请人： Akira Takeda ,  Tomoko Kamei ,  Masao Kageyama ,  Kenzo Motosugi

    发明人： Akira Takeda ,  Tomoko Kamei ,  Masao Kageyama ,  Kenzo Motosugi

    IPC分类号： C12N9/20 ,  C12Q1/44

    CPC分类号： C12Q1/44 ,  C12N9/20 ,  Y10S435/906

    摘要： The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.

    摘要翻译： 本发明涉及能够将脂蛋白中的甘油三酯水解成甘油和脂肪酸的热稳定性脂蛋白脂肪酶，产生热稳定性脂蛋白脂肪酶的方法和含有热稳定性脂蛋白脂肪酶的血液甘油三酯测定试剂。 热稳定性脂蛋白脂肪酶在约60℃左右pH4〜7的缓冲液中处理约15分钟，甘油形成活性/脂肪酸形成活性比为 至少约15％。 该方法包括培养嗜热放线菌，特别是Streptomyces 7825（FERM P-9983，培养FERM BP-2489以产生热稳定性脂蛋白脂肪酶）。

    Acyl-CoA synthetase
    6.
    发明授权
    Acyl-CoA synthetase 失效
    酰基辅酶A合成酶

    公开(公告)号：US4794084A

    公开(公告)日：1988-12-27

    申请号：US11478

    申请日：1987-02-05

    申请人： Mitsuo Watanabe ,  Hiromi Sato ,  Tomoko Kamei ,  Masao Kageyama

    发明人： Mitsuo Watanabe ,  Hiromi Sato ,  Tomoko Kamei ,  Masao Kageyama

    IPC分类号： C12N9/00 ,  C12N1/20

    CPC分类号： C12N9/93 ,  C12R1/38 ,  Y10S435/874

    摘要： An acyl-CoA synthetase is disclosed having the following characteristics:(a) Reaction:Capable of acting on saturated or unsaturated lower to higher fatty acids to produce a CoA derivative thereof (Acyl-CoA), adenosine monophosphate and pyrophosporic acid, in the presence of adenosine triphosphate and coenzyme A;(b) Stability:Having a residual activity of not less than about 50% after being incubated in a buffer (pH about 7.5 for about 15 minutes at a temperature of about 55.degree. C.; and(c) Optimum reaction temperature:Having an optimum reaction temperature in the range of from 50.degree. to 60.degree. C.Also disclosed is Psuedomonas UKSW-3733 strain which is capable of producing the acyl-CoA synthetase, which has an optimum temperature for growth in the range of from 45.degree. to 55.degree. C. and is incapable of assimilating carbonates.The acyl-CoA synthetase is highly stable and, therefore, can be used for preparation of clinical diagnosis kits capable of giving accurate test results over an extended period of time.

    摘要翻译： 公开了具有以下特征的酰基-CoA合成酶：（a）反应：能够在饱和或不饱和的低级至高级脂肪酸上作用，以产生其CoA衍生物（Acyl-CoA），单磷酸腺苷和焦磷酸， 的三磷酸腺苷和辅酶A; （b）稳定性：在缓冲液（pH约7.5左右，在约55℃的温度下约15分钟后）具有不小于约50％的残余活性;和（c）最佳反应温度：具有 50℃至60℃范围内的最佳反应温度。还公开了能够产生酰基辅酶A合成酶的Psuedomonas UKSW-3733菌株，其具有在45℃至55℃范围内生长的最佳温度 并且不能同化碳酸酯，酰基辅酶A合成酶是高度稳定的，因此可用于制备能够在更长时间内提供准确测试结果的临床诊断试剂盒。