公开(公告)号：US07405267B2

公开(公告)日：2008-07-29

申请号：US10621353

申请日：2003-07-18

申请人： Tae-Won Kang ,  Byoung-Taek Choi ,  Gang-Sun Choi ,  Yong-Rack Choi ,  Sung-Ho Hwang

发明人： Tae-Won Kang ,  Byoung-Taek Choi ,  Gang-Sun Choi ,  Yong-Rack Choi ,  Sung-Ho Hwang

IPC分类号： C07K1/14 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C12P19/28 ,  C07K5/12 ,  C07K5/02

CPC分类号： C07K9/008

摘要： Disclosed is a method for purifying teicoplanin A2 comprising: (i) a primary pre-purification step of purifying a filtrate of fermentation broth of a strain using a synthetic adsorbent; (ii) a secondary pre-purification step of purifying the primary pre-purification solution using a cation exchange resin having a high cross-linkage, a catalytic resin or a chelate resin; (iii) a final purification step of purifying the secondary pre-purification solution using a reversed phase resin; and (iv) a powder-forming step. According to the present invention, it is possible to obtain teicoplanin A2 with a higher purity through a relatively simple process without using an excessive amount of an organic solvent.

摘要翻译： 公开了一种纯化泰可菌素A 2 O 2的方法，其包括：（i）使用合成吸附剂纯化菌株发酵液的滤液的初级预纯化步骤; （ii）使用具有高交联键的阳离子交换树脂，催化树脂或螯合树脂来纯化所述初级预纯化溶液的二次预纯化步骤; （iii）使用反相树脂纯化二次预纯化溶液的最终纯化步骤; 和（iv）粉末形成步骤。 根据本发明，可以通过相对简单的方法获得具有较高纯度的替考拉宁A 2 N 2，而不使用过量的有机溶剂。