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公开(公告)号:US4517300A
公开(公告)日:1985-05-14
申请号:US475245
申请日:1983-03-14
申请人: Taichi Manome , Toshinori Ohmine , Takao Okazaki , Mamoru Arai
发明人: Taichi Manome , Toshinori Ohmine , Takao Okazaki , Mamoru Arai
CPC分类号: C12N15/76
摘要: A novel plasmid, named pSAN 181, has a molecular weight of 5.+-.0.2 megadaltons and single cleavage sites for each of the restriction endonucleases BamHI, PstI, XhoI and BglII, the positions of said sites being 0, about 0.85, about 1.15 and about 3.95 megadaltons from the cleavage site of BamHI. The plasmid is extracted from mycelia of microorganisms of the species Streptomyces fulvoviridis, especially the strain Streptomyces fulvoviridis FERM P-6279, and is useful as a vector for genetic engineering.
摘要翻译: 命名为pSAN 181的新型质粒具有5 +/- 0.2兆道尔顿的分子量和每个限制性内切核酸酶BamHI,PstI,XhoI和BglII的单个切割位点,所述位点的位置为0,约0.85,约1.15 和约3.95兆巴斯从BamHI的切割位点。 该质粒是从链霉菌链霉菌(Streptomyces fulvoviridis)的微生物的菌丝体中提取的,特别是菌丝霉菌菌丝霉F-P-6279菌株,可用作基因工程载体。