公开(公告)号：US5521287A

公开(公告)日：1996-05-28

申请号：US202130

申请日：1994-02-25

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

IPC分类号： A61K38/00 ,  C07K14/765 ,  A61K38/38 ,  C07K1/16 ,  C07K1/36

CPC分类号： C07K14/765 ,  A61K2039/545 ,  A61K38/00

摘要： Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. The thus obtained human serum albumin can further be purified by treating recombinant human serum albumin with a hydrophobic chromatography carrier at pH of 2 to 5 and a salt concentration of 0.4 to 1 and exposing the carrier to a pH of 6 to 8 and a salt concentration of 0.01 to 0.3 M, or treating the culture supernatant with boric acid or a salt thereof at pH 8 to 11 for 1 to 10 hours and recovering the supernatant. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

公开(公告)号：US5986062A

公开(公告)日：1999-11-16

申请号：US538471

申请日：1995-10-03

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Furuhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama ,  Nagatoshi Fujiwara

IPC分类号： A61K38/00 ,  C07K14/765

CPC分类号： C07K14/765 ,  A61K2039/545 ,  A61K38/00

摘要： Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. The thus obtained human serum albumin can further be purified by treating recombinant human serum albumin with a hydrophobic chromatography carrier at pH of 2 to 5 and a salt concentration of 0.4 to 1 and exposing the carrier to a pH of 6 to 8 and a salt concentration of 0.01 to 0.3 M, or treating the culture supernatant with boric acid or a salt thereof at pH 8 to 11 for 1 to 10 hours and recovering the supernatant. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

摘要翻译： 通过基因操作技术获得的人血清白蛋白可以通过特定步骤的组合来纯化，其中将得自人血清白蛋白的宿主的培养上清液进行超滤，热处理，酸处理和另一次超滤，然后用 阳离子交换剂，疏水性色谱载体和阴离子交换剂，并通过盐析，从而获得纯的形式的人血清白蛋白，其基本上不含有配制成药物制剂的蛋白质和多糖污染物。 由此获得的人血清白蛋白可以进一步通过用pH为2至5和盐浓度为0.4至1的疏水色谱载体处理重组人血清白蛋白并将载体暴露于pH6至8和盐浓度 为0.01〜0.3M，或用硼酸或其盐在pH8〜11处理培养上清1〜10小时，回收上清液。 该方法使得可以有效地纯化重组人血清白蛋白并提供基本上纯的人血清白蛋白，其不含生产者宿主相关物质和其它污染物，并且没有着色。

公开(公告)号：US5440018A

公开(公告)日：1995-08-08

申请号：US36387

申请日：1993-03-24

申请人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama

发明人： Takao Ohmura ,  Akinori Sumi ,  Wataru Ohtani ,  Naoto Fuluhata ,  Kazuya Takeshima ,  Kaeko Kamide ,  Munehiro Noda ,  Masahide Kondo ,  Syoichi Ishikawa ,  Kazuhiro Oohara ,  Kazumasa Yokoyama

IPC分类号： A61K38/00 ,  C07K1/14 ,  C07K14/765 ,  C12N15/14 ,  A61K38/38 ,  C07K1/16 ,  C07K1/36

CPC分类号： C07K14/765 ,  A61K38/00

摘要： Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

摘要翻译： 通过基因操作技术获得的人血清白蛋白可以通过特定步骤的组合来纯化，其中将得自人血清白蛋白的宿主的培养上清液进行超滤，热处理，酸处理和另一次超滤，然后用 阳离子交换剂，疏水性色谱载体和阴离子交换剂，并通过盐析，从而获得纯的形式的人血清白蛋白，其基本上不含有配制成药物制剂的蛋白质和多糖污染物。 该方法使得可以有效地纯化重组人血清白蛋白并提供基本上纯的人血清白蛋白，其不含生产者宿主相关物质和其它污染物，并且没有着色。

公开(公告)号：US07330442B2

公开(公告)日：2008-02-12

申请号：US10370167

申请日：2003-02-18

申请人： Kazuhiro Oohara

发明人： Kazuhiro Oohara

IPC分类号： H04L12/40

CPC分类号： H04L12/40078 ,  H04L12/40117 ,  H04L12/403 ,  H04L12/6418 ,  H04L41/00

摘要： A communication apparatus as a node of a communication network comprises a reading device that reads management information representing management status of a dominated node stored in the dominated node connected via the communication network, a writing device that writes identification information for managing the dominated node into the dominated node when the management information does not include identification information of other dominating node, and a management device that manages the dominated node. The communication apparatus is complied with the IEEE 1394 Standard and capable of managing the other nodes on the communication network.

摘要翻译： 作为通信网络的节点的通信装置包括读取装置，其读取表示存储在经由通信网络连接的主导节点中的主导节点的管理状态的管理信息;写入装置，其将用于管理主导节点的识别信息写入到 管理信息不包含其他控制节点的识别信息的管理节点，以及管理主导节点的管理装置。 通信装置符合IEEE 1394标准，并能够管理通信网络上的其他节点。

公开(公告)号：US07542434B2

公开(公告)日：2009-06-02

申请号：US11881985

申请日：2007-07-27

申请人： Kazuhiro Oohara

发明人： Kazuhiro Oohara

IPC分类号： H04L12/40

CPC分类号： H04L12/40078 ,  H04L12/40117 ,  H04L12/403 ,  H04L12/6418 ,  H04L41/00

摘要： A communication apparatus as a node of a communication network comprises a reading device that reads management information representing management status of a dominated node stored in the dominated node connected via the communication network, a writing device that writes identification information for managing the dominated node into the dominated node when the management information does not include identification information of other dominating node, and a management device that manages the dominated node. The communication apparatus is complied with the IEEE 1394 Standard and capable of managing the other nodes on the communication network.

摘要翻译： 作为通信网络的节点的通信装置包括读取装置，其读取表示存储在经由通信网络连接的主导节点中的主导节点的管理状态的管理信息;写入装置，其将用于管理主导节点的识别信息写入到 管理信息不包含其他控制节点的识别信息的管理节点，以及管理主导节点的管理装置。 通信装置符合IEEE 1394标准，并能够管理通信网络上的其他节点。

公开(公告)号：US20070274319A1

公开(公告)日：2007-11-29

申请号：US11881985

申请日：2007-07-27

申请人： Kazuhiro Oohara

发明人： Kazuhiro Oohara

IPC分类号： H04L12/56

CPC分类号： H04L12/40078 ,  H04L12/40117 ,  H04L12/403 ,  H04L12/6418 ,  H04L41/00

摘要： A communication apparatus as a node of a communication network comprises a reading device that reads management information representing management status of a dominated node stored in the dominated node connected via the communication network, a writing device that writes identification information for managing the dominated node into the dominated node when the management information does not include identification information of other dominating node, and a management device that manages the dominated node. The communication apparatus is complied with the IEEE1394 Standard and capable of managing the other nodes on the communication network.

摘要翻译： 作为通信网络的节点的通信装置包括读取装置，其读取表示存储在经由通信网络连接的主导节点中的主导节点的管理状态的管理信息;写入装置，其将用于管理主导节点的识别信息写入到 管理信息不包含其他控制节点的识别信息的管理节点，以及管理主导节点的管理装置。 通信装置符合IEEE1394标准，并且能够管理通信网络上的其他节点。

公开(公告)号：US06189040B1

公开(公告)日：2001-02-13

申请号：US08906565

申请日：1997-08-05

申请人： Kazuhiro Oohara

发明人： Kazuhiro Oohara

IPC分类号： G06F1300

CPC分类号： H04L29/06

摘要： A data relaying unit includes a first input/output terminal for connection to a host device, a plurality of second input/output terminals for connection with a plurality of client devices, a quasi host device that, in accordance with an instruction from the host computer, controls direct data transmission between the client devices, and a connection switching device that selectively connects the individually second input/output terminals to either the first input/output terminal or the quasi host device. In accordance with an instruction from the host computer, the connection switching device connects the second input/output terminals of predetermined ones of the client devices to the quasi host device so as to permit direct data transmission between the predetermined client devices. Schedule for the data transmission between the client devices is managed by the quasi host device. The quasi host may monitor a condition of the data transmission between the client devices and inform the host device of presence/absence of a unusual condition or the like.

摘要翻译： 数据中继单元包括用于连接到主机设备的第一输入/输出端子，用于与多个客户端设备连接的多个第二输入/输出端子，准主机设备，其根据来自主计算机的指令 控制客户端设备之间的直接数据传输，以及选择性地将第二输入/输出终端连接到第一输入/输出终端或准主机设备的连接切换设备。 根据来自主计算机的指令，连接切换装置将预定的客户机装置的第二输入/输出端子连接到准主机装置，以便允许在预定的客户装置之间进行直接的数据传输。 客户端设备之间数据传输的时间表由准主机设备进行管理。 准主机可以监视客户端设备之间的数据传输的状况，并通知主机设备存在/不存在异常情况等。