公开(公告)号：US20080280304A1

公开(公告)日：2008-11-13

申请号：US12139701

申请日：2008-06-16

申请人： Takashi Hirao ,  Masayuki Hiramoto

发明人： Takashi Hirao ,  Masayuki Hiramoto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895

摘要： A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genusThe method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genus Fagopyrum, can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.

摘要翻译： 一种用于检测目标植物属物种的方法，包括以下步骤：使用选自引物（A）和（B）中的至少一种成员进行PCR，其可在严格条件下与具有共同的核酸分子杂交 在45S rRNA前体基因序列中的目标植物属中的所有物种的核苷酸序列，其中引物（A）的3'末端可以在引物与核酸杂交时与靶植物属的ITS-1序列中的碱基互补结合 酸分子，而当引物与核酸分子杂交时，引物（B）的3'末端可以与靶植物属的ITS-2序列中的碱基互补结合，并且从PCR中鉴定所得扩增产物的存在， 目标植物属的ITS-1或ITS-2序列的至少一部分目标植物属，特别是过敏原植物属如禾本科属的物种的检测方法可以使其成为pos 可以以高灵敏度检测，例如在植物包含在食物成分或食品中的情况下，约1ppm的植物。

公开(公告)号：US20070048779A1

公开(公告)日：2007-03-01

申请号：US11581872

申请日：2006-10-17

申请人： Takashi Hirao ,  Masayuki Hiramoto

发明人： Takashi Hirao ,  Masayuki Hiramoto

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895

摘要： A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genus. The method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genus Fagopyrum, can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.

摘要翻译： 一种用于检测目标植物属物种的方法，包括以下步骤：使用选自引物（A）和（B）中的至少一种成员进行PCR，其可在严格条件下与具有共同的核酸分子杂交 在45S rRNA前体基因序列中的目标植物属中的所有物种的核苷酸序列，其中引物（A）的3'末端可以在引物与核酸杂交时与靶植物属的ITS-1序列中的碱基互补结合 酸分子，而当引物与核酸分子杂交时，引物（B）的3'末端可以与靶植物属的ITS-2序列中的碱基互补结合，并且从PCR中鉴定所得扩增产物的存在， 目标植物属的ITS-1或ITS-2序列的至少一部分。 用于检测目标植物属，特别是变应原植物属（如禾本科）的物种的方法可以使得可以以高灵敏度检测例如约1ppm的植物（例如植物（ s）包含在食品成分或食品中。

公开(公告)号：US07144702B2

公开(公告)日：2006-12-05

申请号：US10285061

申请日：2002-10-31

申请人： Takashi Hirao ,  Masayuki Hiramoto

发明人： Takashi Hirao ,  Masayuki Hiramoto

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895

摘要： A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genus.The method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genus Fagopyrum, can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.

摘要翻译： 一种用于检测目标植物属物种的方法，包括以下步骤：使用选自引物（A）和（B）中的至少一种成员进行PCR，其可在严格条件下与具有共同的核酸分子杂交 在45S rRNA前体基因序列中的目标植物属中的所有物种的核苷酸序列，其中引物（A）的3'末端可以在引物与核酸杂交时与靶植物属的ITS-1序列中的碱基互补结合 酸分子，而当引物与核酸分子杂交时，引物（B）的3'末端可以与靶植物属的ITS-2序列中的碱基互补结合，并且从PCR中鉴定所得扩增产物的存在， 目标植物属的ITS-1或ITS-2序列的至少一部分。 用于检测目标植物属，特别是变应原植物属（如禾本科）的物种的方法可以使得可以以高灵敏度检测例如约1ppm的植物（例如植物（ s）包含在食品成分或食品中。

公开(公告)号：US07704694B2

公开(公告)日：2010-04-27

申请号：US12139701

申请日：2008-06-16

申请人： Takashi Hirao ,  Masayuki Hiramoto

发明人： Takashi Hirao ,  Masayuki Hiramoto

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895

摘要： A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genusThe method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genus Fagopyrum, can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.

摘要翻译： 一种用于检测目标植物属物种的方法，包括以下步骤：使用选自引物（A）和（B）中的至少一种成员进行PCR，其可在严格条件下与具有共同的核酸分子杂交 在45S rRNA前体基因序列中的目标植物属中的所有物种的核苷酸序列，其中引物（A）的3'末端可以在引物与核酸杂交时与靶植物属的ITS-1序列中的碱基互补结合 酸分子，而当引物与核酸分子杂交时，引物（B）的3'末端可以与靶植物属的ITS-2序列中的碱基互补结合，并且从PCR中鉴定所得扩增产物的存在， 目标植物属的ITS-1或ITS-2序列的至少一部分目标植物属，特别是过敏原植物属如禾本科属的物种的检测方法可以使其 可能以高灵敏度检测，例如在植物包含在食品成分或食品中的情况下，约1ppm的植物。

公开(公告)号：US07402391B2

公开(公告)日：2008-07-22

申请号：US11581872

申请日：2006-10-17

申请人： Takashi Hirao ,  Masayuki Hiramoto

发明人： Takashi Hirao ,  Masayuki Hiramoto

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895

摘要： A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genus.The method for detecting species in a target plant genus, particularly an allergenic plant genus such as the genus Fagopyrum, can make it possible to detect with high sensitivity, for example, about 1 ppm of the plant(s) in cases where the plant(s) is contained in a food ingredient or food product.

摘要翻译： 一种用于检测目标植物属物种的方法，包括以下步骤：使用选自引物（A）和（B）中的至少一种成员进行PCR，其可在严格条件下与具有共同的核酸分子杂交 在45S rRNA前体基因序列中的目标植物属中的所有物种的核苷酸序列，其中引物（A）的3'末端可以在引物与核酸杂交时与靶植物属的ITS-1序列中的碱基互补结合 酸分子，而当引物与核酸分子杂交时，引物（B）的3'末端可以与靶植物属的ITS-2序列中的碱基互补结合，并且从PCR中鉴定所得扩增产物的存在， 目标植物属的ITS-1或ITS-2序列的至少一部分。 用于检测目标植物属，特别是变应原植物属（如禾本科）的物种的方法可以使得可以以高灵敏度检测例如约1ppm的植物（例如植物（ s）包含在食品成分或食品中。

公开(公告)号：US20080064028A1

公开(公告)日：2008-03-13

申请号：US10556903

申请日：2004-05-14

申请人： Takashi Hirao ,  Masayuki Hiramoto ,  Satoshi Watanabe ,  Jinji Shono

发明人： Takashi Hirao ,  Masayuki Hiramoto ,  Satoshi Watanabe ,  Jinji Shono

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C12Q1/686 ,  C12Q2561/113

摘要： Provided is a method of quantifying a specific plant genus in a food or a food ingredient by a PCR method, comprising: (i) preparing a sample for correction where a sample derived from the specific plant genus to be detected and a standard plant sample are mixed in a predetermined ratio, and extracting genomic DNA from the sample for correction; (ii) preparing a test sample where a known amount of the standard plant sample is added to the food or the food ingredient to be examined, and extracting genomic DNA from the test sample; (iii) practicing a quantitative PCR method using the genomic DNAs and primers; and (iv) conducting correction with a standard value for correction determined for the sample for correction to calculate the amount of the specific plant ingredient contained in the test sample.

摘要翻译： 本发明提供了一种通过PCR方法对食品或食品成分中的特定植物属进行定量的方法，其特征在于，包括：（i）制备用于校正的样品，其中来源于要检测的特定植物属的样品和标准植物样品为 以预定比例混合，并从样品中提取基因组DNA进行校正; （ii）制备测试样品，其中将已知量的标准植物样品加入到待检查的食品或食品成分中，并从测试样品中提取基因组DNA; （iii）使用基因组DNA和引物进行定量PCR方法; 和（iv）进行用于校正的样品的校正标准值的校正，以计算测试样品中包含的特定植物成分的量。

公开(公告)号：USD452845S1

公开(公告)日：2002-01-08

申请号：US29132847

申请日：2000-11-17

申请人： Masayuki Hiramoto ,  Takashi Suyama

设计人： Masayuki Hiramoto ,  Takashi Suyama

公开(公告)号：USD452479S1

公开(公告)日：2001-12-25

申请号：US29132817

申请日：2000-11-17

申请人： Masayuki Hiramoto ,  Takashi Suyama

设计人： Masayuki Hiramoto ,  Takashi Suyama

公开(公告)号：USD452478S1

公开(公告)日：2001-12-25

申请号：US29132815

申请日：2000-11-17

申请人： Masayuki Hiramoto ,  Takashi Suyama

设计人： Masayuki Hiramoto ,  Takashi Suyama

公开(公告)号：US07056146B2

公开(公告)日：2006-06-06

申请号：US11097135

申请日：2005-04-04

申请人： Masayuki Hiramoto ,  Takashi Suyama ,  Terumi Nakashima

发明人： Masayuki Hiramoto ,  Takashi Suyama ,  Terumi Nakashima

IPC分类号： H01R14/24

CPC分类号： H01R12/79 ,  H01R4/2454 ,  H01R12/716 ,  H01R43/16

摘要： An insulation displacement contact includes a pair of insulation displacement blades and a pair of resilient contact pieces. The pair of insulation displacement blades are opposite to each other with their bases connected to each other such that there is formed, by their inner sides, a slot for receiving an insulated wire of which core wire portion is covered with an insulation. The pair of insulation displacement blades are arranged such that when the insulated wire is inserted into the slot, the insulation is cut and the core wire portion comes in press-contact with the insulation displacement blades. Each of the pair of resilient contact pieces is made of a plate member which is connected to the outer side of each insulation displacement blade, which extends toward the side opposite of the inlet of the slot up to a position exceeding the base of each insulation displacement blade, which has a contact portion for holding or nipping a mating contact at a position opposite of the slot inlet with respect to the base of each insulation displacement blade, and which has, between the connection portion connected to the outer side of each insulation displacement blade and the contact portion, a tapering portion of which width is gradually narrowed in the direction toward the contact portion.

摘要翻译： 绝缘位移触点包括一对绝缘移动叶片和一对弹性接触件。 一对绝缘位移叶片彼此相对，其底部彼此连接，使得它们的内侧形成有用于接收芯线部分被绝缘层覆盖的绝缘线的槽。 一对绝缘位移叶片被布置成使得当绝缘线插入槽中时，绝缘被切割并且芯线部分与绝缘置换叶片压接。 一对弹性接触片中的每一个由板构件构成，该板构件连接到每个绝缘置换叶片的外侧，该绝缘位移叶片朝向与槽的入口相反的一侧延伸到超过每个绝缘位移的基部的位置 叶片，其具有用于在相对于每个绝缘置换叶片的基部的槽入口相对的位置处保持或夹持配合接触件的接触部分，并且其在连接到每个绝缘位移的外侧的连接部分之间 叶片和接触部分，其锥形部分的宽度在朝向接触部分的方向上逐渐变窄。