公开(公告)号：US20100075305A1

公开(公告)日：2010-03-25

申请号：US12311217

申请日：2007-09-26

申请人： Takayuki Ezaki ,  Kiyofumi Ohkusu ,  Shigeru Yoshida

发明人： Takayuki Ezaki ,  Kiyofumi Ohkusu ,  Shigeru Yoshida

IPC分类号： C12Q1/68 ,  C12Q1/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/04 ,  C12Q2600/16 ,  G01N33/56911 ,  Y02A50/451 ,  Y02A50/52

摘要： Disclosed is a method for detecting the species of a bacterium in a simple manner. Specifically disclosed is a method for detecting the species of a bacterium, which can detect at least one bacterial species selected from Staphylococcus species, Streptococcus species, Klebsiella species, Escherichia species, Mycobacterium species, Legionella species, Vibrio species, Bacillus species, Neisseria species, Campylobacter species, Chlamydia species, Chlamydophila species, Mycoplasma species, Listeria species, Salmonella species and Yersinia species, and which comprises the steps of: a) contacting a sample suspected to contain a nucleic acid derived from the bacterial species; and b) detecting the presence or absence of the hybridization between the nucleic acid molecule and the nucleic acid contained in the sample.

摘要翻译： 公开了以简单的方式检测细菌的种类的方法。 具体公开了一种检测细菌种的方法，该细菌可以检测至少一种选自葡萄球菌属，链球菌属，克雷伯菌属，埃希氏杆菌属，分枝杆菌属，军团菌属，弧菌属，芽孢杆菌属，奈瑟氏球菌属， 弯曲杆菌属，衣原体属，衣原体属，支原体属，利斯特氏菌属，沙门氏菌属和耶尔森氏菌属，包括以下步骤：a）使疑似含有源自细菌种的核酸的样品接触; 和b）检测核酸分子与样品中所含的核酸之间是否存在杂交。