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    METHODS FOR PRODUCING ANTIBODY-PRODUCING CELLS THAT PRODUCE DESIRED POLYPEPTIDES
    7.
    发明申请
    METHODS FOR PRODUCING ANTIBODY-PRODUCING CELLS THAT PRODUCE DESIRED POLYPEPTIDES 审中-公开
    用于生产产生所需多肽的抗体产生细胞的方法

    公开(公告)号：US20130071881A1

    公开(公告)日：2013-03-21

    申请号：US13510910

    申请日：2010-11-18

    申请人： Hitoshi Ohmori ,  Naoki Kanayama ,  Shinobu Fujii

    发明人： Hitoshi Ohmori ,  Naoki Kanayama ,  Shinobu Fujii

    IPC分类号： C12N15/66

    CPC分类号： C12N15/66 ,  C07K16/462 ,  C12N15/907 ,  C12N2800/30

    摘要： An objective of the present invention is to provide methods for introducing DNAs encoding a desired amino acid sequence into a region comprising a DNA encoding an antibody variable region of antibody-producing cells. The present inventors developed methods for efficiently introducing DNAs encoding a desired amino acid sequence into the antibody variable region gene locus of DT40-SW, which is a mutant line of the DT40 chicken B cell line which has the ability to spontaneously introduce mutations. This allows mutagenesis of introduced DNAs to modify the polypeptides to have superior functions. In particular, the present inventors revealed the nucleotide sequence of the antibody H chain variable region gene locus of the DT40 cell line. Based on this finding, the present inventors successfully constructed targeting vectors that allow efficient substitution of the antibody H chain variable region gene locus of the DT40 cell line with a gene encoding a desired polypeptide.

    摘要翻译： 本发明的目的是提供将编码期望的氨基酸序列的DNA导入包含编码抗体产生细胞的抗体可变区的DNA的区域的方法。 本发明人开发了将编码期望的氨基酸序列的DNA有效地引入到具有自发引入突变能力的DT40鸡B细胞系的突变体系DT40-SW的抗体可变区基因座中的方法。 这允许引入的DNA的突变来修饰多肽以具有优异的功能。 具体而言，本发明人揭示了DT40细胞系的抗体H链可变区基因座的核苷酸序列。 基于这一发现，本发明人成功地构建了能够用编码所需多肽的基因有效取代DT40细胞系的抗体H链可变区基因座的靶向载体。