公开(公告)号：US07074903B2

公开(公告)日：2006-07-11

申请号：US10424726

申请日：2003-04-29

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： C07K16/40 ,  C12N5/20 ,  C12P21/08 ,  G01N33/543 ,  G01N33/573

CPC分类号： C07K16/40 ,  Y10S435/96 ,  Y10S435/975

摘要： Monocolonal antibodies having a higher reactivity with tartrate-resistant acid phosphatase 5b (TRACP 5b) than tartrate-resistant acid phosphatase 5a (TRACP 5a) and having a higher specificity to TRACP 5b can be obtained by cell fusion using as antigens TRACP 5b purified from human osteoclasts. By using the monoclonal antibody, TRACP 5b in a sample can be detected specifically with a high sensitivity.

公开(公告)号：US07611855B2

公开(公告)日：2009-11-03

申请号：US10538912

申请日：2003-12-24

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

IPC分类号： G01N33/543 ,  G01N33/546 ,  G01N33/573 ,  C07K16/40 ,  C12P21/08 ,  C12N5/20

CPC分类号： G01N33/54393 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/811

摘要： Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.

摘要翻译： 使用两种类型的抗体，即对靶物质的亲和力高于竞争性物质的第一抗体和对于竞争性物质具有比目标物质更高的亲和力的第二抗体，用这两种抗体 。 然后，样品中的竞争性物质首先与第二抗体结合，从而扩大了样品中目标物质与竞争性物质的比例。 结果，与仅使用第一抗体的情况相比，靶物质变得易于与第一抗体结合，并且反过来，靶物质的反应性升高。 因此，可以准确地测定样品中的目标物质，同时避免样品中所含的竞争性物质的影响。