公开(公告)号：US07611855B2

公开(公告)日：2009-11-03

申请号：US10538912

申请日：2003-12-24

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

IPC分类号： G01N33/543 ,  G01N33/546 ,  G01N33/573 ,  C07K16/40 ,  C12P21/08 ,  C12N5/20

CPC分类号： G01N33/54393 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/811

摘要： Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.

摘要翻译： 使用两种类型的抗体，即对靶物质的亲和力高于竞争性物质的第一抗体和对于竞争性物质具有比目标物质更高的亲和力的第二抗体，用这两种抗体 。 然后，样品中的竞争性物质首先与第二抗体结合，从而扩大了样品中目标物质与竞争性物质的比例。 结果，与仅使用第一抗体的情况相比，靶物质变得易于与第一抗体结合，并且反过来，靶物质的反应性升高。 因此，可以准确地测定样品中的目标物质，同时避免样品中所含的竞争性物质的影响。

公开(公告)号：US07074903B2

公开(公告)日：2006-07-11

申请号：US10424726

申请日：2003-04-29

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： C07K16/40 ,  C12N5/20 ,  C12P21/08 ,  G01N33/543 ,  G01N33/573

CPC分类号： C07K16/40 ,  Y10S435/96 ,  Y10S435/975

摘要： Monocolonal antibodies having a higher reactivity with tartrate-resistant acid phosphatase 5b (TRACP 5b) than tartrate-resistant acid phosphatase 5a (TRACP 5a) and having a higher specificity to TRACP 5b can be obtained by cell fusion using as antigens TRACP 5b purified from human osteoclasts. By using the monoclonal antibody, TRACP 5b in a sample can be detected specifically with a high sensitivity.

公开(公告)号：US20090275059A1

公开(公告)日：2009-11-05

申请号：US12379904

申请日：2009-03-04

申请人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

发明人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

IPC分类号： G01N33/566

CPC分类号： G01N33/6893 ,  G01N2333/75 ,  G01N2333/775 ,  G01N2800/08

摘要： Using the protein chip technology, biological samples such as sera are subjected to proteome analysis. Thus, a protein which is a human fibrinogen α-E chain decomposition product and has a molecular weight of 5,900, a protein which is an apolipoprotein AII decomposition product and has a molecular weight of 7,800, and a protein which is an apolipoprotein AI decomposition product and has a molecular weight of 28,000, each showing an increase or a decrease with the habit of drinking, are newly found out. By detecting or quantifying these proteins, a liver disease in a subject such as one having a problem of drinking can be diagnosed at the early stage.

公开(公告)号：US07517951B2

公开(公告)日：2009-04-14

申请号：US10538916

申请日：2003-12-24

申请人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

发明人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

IPC分类号： A61K38/00 ,  A61K35/14

CPC分类号： G01N33/6893 ,  G01N2333/75 ,  G01N2333/775 ,  G01N2800/08

摘要： Using the protein chip technology, biological samples such as sera are subjected to proteome analysis. Thus, a protein which is a human fibrinogen α-E chain decomposition product and has a molecular weight of 5,900, a protein which is an apolipoprotein AII decomposition product and has a molecular weight of 7,800, and a protein which is an apolipoprotein AI decomposition product and has a molecular weight of 28,000, each showing an increase or a decrease with the habit of drinking, are newly found out. By detecting or quantifying these proteins, a liver disease in a subject such as one having a problem of drinking can be diagnosed at the early stage.

摘要翻译： 使用蛋白质芯片技术，生物样品如血清进行蛋白质组分析。 因此，作为人纤维蛋白原α-E链分解产物的分子量为5900的蛋白质，作为载脂蛋白AII分解产物，分子量为7800的蛋白质，以及作为载脂蛋白AI分解产物的蛋白质 并且新发现的分子量为28,000，分别显示饮酒习惯的增加或减少。 通过检测或定量这些蛋白质，可以在早期诊断患有饮酒问题的受试者的肝脏疾病。

公开(公告)号：US07781180B2

公开(公告)日：2010-08-24

申请号：US12379904

申请日：2009-03-04

申请人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

发明人： Fumio Nomura ,  Kazuyuki Sogawa ,  Takeshi Tomonaga ,  Takenori Ochiai ,  Hideaki Shimada ,  Tatsuya Ohashi ,  Katsuhiro Katayama

IPC分类号： A61K38/00 ,  A61K35/14 ,  G01N33/53

CPC分类号： G01N33/6893 ,  G01N2333/75 ,  G01N2333/775 ,  G01N2800/08

摘要： Using the protein chip technology, biological samples such as sera are subjected to proteome analysis. Thus, a protein which is a human fibrinogen α-E chain decomposition product and has a molecular weight of 5,900, a protein which is an apolipoprotein AII decomposition product and has a molecular weight of 7,800, and a protein which is an apolipoprotein AI decomposition product and has a molecular weight of 28,000, each showing an increase or a decrease with the habit of drinking, are newly found out. By detecting or quantifying these proteins, a liver disease in a subject such as one having a problem of drinking can be diagnosed at the early stage.

摘要翻译： 使用蛋白质芯片技术，生物样品如血清进行蛋白质组分析。 因此，作为人纤维蛋白原α-E链分解产物，分子量为5900的蛋白质，作为载脂蛋白AII分解产物，分子量为7800的蛋白质，以及作为载脂蛋白AI分解产物的蛋白质 并且新发现的分子量为28,000，分别显示饮酒习惯的增加或减少。 通过检测或定量这些蛋白质，可以在早期诊断患有饮酒问题的受试者的肝脏疾病。

公开(公告)号：US07465556B2

公开(公告)日：2008-12-16

申请号：US10546608

申请日：2004-02-20

申请人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： C12Q1/42 ,  G01N33/573 ,  G01N33/53 ,  G01N33/535

CPC分类号： G01N33/573 ,  Y10S436/815

摘要： Specific and accurate immunoassay of tartrate resistant acid phosphatase 5b (TRACP 5b) in a specimen may be carried out by bonding TRACP 5b in the specimen to an antibody, subjecting the TRACP 5b bonded to the antibody to enzyme reaction with a 2-halo-4-nitrophenylphosphoric acid or a salt thereof, a substrate for TRACP 5b, and then assaying the enzymatic activity of TRACP 5b.

摘要翻译： 样品中酒石酸盐酸性磷酸酶5b（TRACP 5b）的特异性和准确的免疫测定可以通过将样品中的TRACP 5b结合到抗体上进行，将与抗体结合的TRACP 5b与2-卤代-4 - 硝基苯基磷酸或其盐，TRACP 5b的底物，然后测定TRACP 5b的酶活性。

公开(公告)号：US20060154317A1

公开(公告)日：2006-07-13

申请号：US10546608

申请日：2004-02-20

申请人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/573 ,  Y10S436/815

摘要： Specific and accurate immunoassay of tartrate resistant acid phosphatase 5b (TRACP 5b) in a specimen may be carried out by bonding TRACP 5b in the specimen to an antibody, subjecting the TRACP 5b bonded to the antibody to enzyme reaction with a 2-halo-4-nitrophenylphosphoric acid or a salt thereof, a substrate for TRACP 5b, and then assaying the enzymatic activity of TRACP 5b.

摘要翻译： 样品中酒石酸盐酸性磷酸酶5b（TRACP 5b）的特异性和准确的免疫测定可以通过将样品中的TRACP 5b结合到抗体上进行，将与抗体结合的TRACP 5b与2-卤代-4 - 硝基苯基磷酸或其盐，TRACP 5b的底物，然后测定TRACP 5b的酶活性。

公开(公告)号：US20110239313A1

公开(公告)日：2011-09-29

申请号：US12521701

申请日：2007-12-28

申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

IPC分类号： C12P21/00 ,  C12N15/85 ,  A01K67/04

CPC分类号： C12N9/16

摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b由蚕生产。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

公开(公告)号：US08426674B2

公开(公告)日：2013-04-23

申请号：US12521701

申请日：2007-12-28

申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

IPC分类号： A01K67/04

CPC分类号： C12N9/16

摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b是从蚕生产的。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

公开(公告)号：US08952215B2

公开(公告)日：2015-02-10

申请号：US12090702

申请日：2006-10-18

申请人： Toshiki Tamura ,  Isao Kobayashi ,  Toshio Kanda ,  Keiro Uchino ,  Katsuhiro Katayama ,  Tatsuya Ohashi ,  Iwao Kiyokawa ,  Hisae Arai ,  Noriyuki Funahashi

发明人： Toshiki Tamura ,  Isao Kobayashi ,  Toshio Kanda ,  Keiro Uchino ,  Katsuhiro Katayama ,  Tatsuya Ohashi ,  Iwao Kiyokawa ,  Hisae Arai ,  Noriyuki Funahashi

IPC分类号： C12N15/00 ,  C07H21/04 ,  C07K16/00 ,  A01K67/033 ,  A01K67/04 ,  C07K14/435 ,  C12N15/85

CPC分类号： C07K16/00 ,  A01K67/0335 ,  A01K67/04 ,  A01K2217/05 ,  A01K2227/703 ,  A01K2267/01 ,  C07K14/43586 ,  C07K2317/10 ,  C07K2317/20 ,  C07K2319/02 ,  C12N15/8509 ,  C12N2830/002 ,  C12N2830/003

摘要： The present inventors produced transgenic silkworms which comprise a promoter of a DNA encoding a protein specifically expressed in the silk gland and a DNA encoding a recombinant antibody whose expression is regulated directly or indirectly by the promoter, and which secrete the recombinant antibody into the silk gland. The recombinant antibodies produced from the silk gland of the transgenic silkworms were confirmed to be active.

摘要翻译： 本发明人生产了转基因蚕，其包含编码丝腺特异性表达的蛋白质的DNA的启动子和编码重组抗体的DNA，其表达由启动子直接或间接调节，并将重组抗体分泌到丝腺中 。 从转基因蚕丝腺产生的重组抗体被证实是活性的。