公开(公告)号：US20050067280A1

公开(公告)日：2005-03-31

申请号：US10674955

申请日：2003-09-30

申请人： Terence Reid ,  Shridhara Karika ,  Milind Nagale ,  Yi Wang ,  Gurdial Sanghera

发明人： Terence Reid ,  Shridhara Karika ,  Milind Nagale ,  Yi Wang ,  Gurdial Sanghera

IPC分类号： G01N27/403 ,  G01N27/26

CPC分类号： G01N27/3272

摘要： An electrochemical cell for detection and quantification of analytes in a liquid sample, particularly a liquid sample having a small volume. In a preferred embodiment, the electrochemical cell comprises an assembly of conducting layers and insulating layers. The electrochemical cell can be formed by depositing conducting materials and insulating materials in alternating layers on an insulating substrate. It is preferred that the layer furthest from the insulating substrate be an insulating layer to minimize the damage of the conducting layers during handling of the electrochemical cell. A passage can be formed through the conducting layers and the insulating layers to expose the edges of the layers, which collectively form the wall or walls of the passage. The exposed edges of the conducting layers form the electrodes of the electrochemical cell. The electrochemical cell comprises at least one working electrode and at least one other electrode, e.g., a dual-purpose reference/counter electrode. In another embodiment, the assembly of conducting layers and insulating layers can be formed on both major surfaces of the insulating substrate. The assembly can comprise at least one working electrode and at least one other electrode, e.g., a dual-purpose reference/counter electrode.

摘要翻译： 一种电化学电池，用于检测和定量液体样品中的分析物，特别是具有小体积的液体样品。 在优选实施例中，电化学电池包括导电层和绝缘层的组件。 电化学电池可以通过在绝缘基板上交替地沉积导电材料和绝缘材料来形成。 优选地，离绝缘基板最远的层是绝缘层，以在处理电化学电池期间最小化导电层的损伤。 可以通过导电层和绝缘层形成通道以暴露层的边缘，这些边缘共同形成通道的壁或壁。 导电层的暴露边缘形成电化学电池的电极。 电化学电池包括至少一个工作电极和至少一个其它电极，例如双用途参考/对电极。 在另一个实施例中，导电层和绝缘层的组合可以形成在绝缘基板的两个主表面上。 该组件可以包括至少一个工作电极和至少一个其它电极，例如双用途参考/对电极。

公开(公告)号：US20050067277A1

公开(公告)日：2005-03-31

申请号：US10674695

申请日：2003-09-30

申请人： Robin Pierce ,  Shridhara Karika ,  Milind Nagale ,  Ross Meyer ,  W. Scott ,  Gurdial Sanghera

发明人： Robin Pierce ,  Shridhara Karika ,  Milind Nagale ,  Ross Meyer ,  W. Scott ,  Gurdial Sanghera

IPC分类号： C12Q1/00 ,  G01N27/26 ,  G01N33/487

CPC分类号： C12Q1/004

摘要： A biosensor in which at least one reagent constitutes a portion of a working electrode, a conductive track leading from a working electrode to an electrical contact associated with a working electrode, or an electrical contact associated with a working electrode. For example, the biosensor can have a mediator or an enzyme or both incorporated into the working electrode itself. Other reagents can be dispensed on the electrode itself either directly or by impregnating a matrix, such as a mesh or a membrane, with the enzyme, and then placing the impregnated mesh or membrane over the electrode. Alternatively, the biosensor can have a mediator or an enzyme or both incorporated into the conductive track leading from the working electrode to an electrical contact associated with the working electrode. In another alternative, the biosensor can have a mediator or an enzyme or both incorporated into the electrical contact associated with the working electrode itself. Furthermore, the biosensor can have a mediator or an enzyme or both incorporated into at least two of the foregoing components of the biosensor.

摘要翻译： 一种生物传感器，其中至少一个试剂构成工作电极的一部分，从工作电极引导到与工作电极相关联的电接触的导电轨道或与工作电极相关联的电接触。 例如，生物传感器可以具有引入工作电极本身的介体或酶或两者。 其他试剂可直接分配在电极本身上，也可以通过用酶浸渍诸如网状物或膜的基质，然后将浸渍的网状物或膜放置在电极上。 或者，生物传感器可以具有引入到从工作电极引导到与工作电极相关联的电接触的导电轨道中的介体或酶或两者。 在另一个替代方案中，生物传感器可以具有介质或酶或两者结合到与工作电极本身相关联的电接触中。 此外，生物传感器可以具有引入到生物传感器的至少两个前述组分中的介体或酶或两者。