专利检索 ap:("The Broad Institute, Inc." OR "Massachusetts Institute of Technology" OR "The Brigham AND Women's Hospital, Inc." OR "President AND Fellows of Harvard College") AND inv:"Jonathan Yee-Ting Flsu" 第 1 页

1.

发明申请
SYSTEMS AND METHODS FOR PREDICTING REPAIR OUTCOMES IN GENETIC ENGINEERING 有权

公开(公告)号：US20220238182A1

公开(公告)日：2022-07-28

申请号：US16772747

申请日：2018-12-15

申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , The Brigham and Women's Hospital, Inc. , President and Fellows of Harvard College

发明人： Max Walt Shen , Jonathan Yee-Ting Flsu , Mandana Arbab , David K. Gifford , David R. Liu , Richard Irving Sherwood

IPC分类号： G16B20/30 , C12N15/10 , G16B40/20 , C12N9/22 , C12N15/11 , A61K38/46 , A61K31/7088

摘要： The specification provides methods for introducing a desired genetic change in a nucleotide sequence using a double-strand break (DSB)-inducing genome editing system, the method comprising: identifying one or more available cut sites in a nucleotide sequence; analyzing the nucleotide sequence and available cut sites with a computational model to identify the optimal cut site for introducing the desired genetic change into the nucleotide sequence; and contacting the nucleotide sequence with a DSB-inducing genome editing system, thereby introducing the desired genetic change in the nucleotide sequence at the cut site.