公开(公告)号：US10571400B2

公开(公告)日：2020-02-25

申请号：US15556998

申请日：2016-03-11

Applicant: The General Hospital Corporation

Inventor： Conor L. Evans ,  W. Peter Hansen ,  Robert A. Hoffman ,  Petra B. Krauledat ,  Daniel W. Cramer

IPC: G01N21/64 ,  G01N15/14 ,  G01N33/58 ,  G01N33/543 ,  C12N5/09 ,  G01N33/569 ,  C12N5/0784 ,  A61K31/7088 ,  A61K31/7105 ,  C12N15/113 ,  A61K31/713 ,  G01N15/00

Abstract: A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.

公开(公告)号：US20180067049A1

公开(公告)日：2018-03-08

申请号：US15556998

申请日：2016-03-11

Applicant: The General Hospital Corporation

Inventor： Conor L. Evans ,  W. Peter Hansen ,  Robert A. Hoffman ,  Petra B. Krauledat ,  Daniel W. Cramer

IPC: G01N21/64 ,  G01N15/14 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58 ,  C12N5/09

Abstract: A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.