公开(公告)号：US20150133434A1

公开(公告)日：2015-05-14

申请号：US14385475

申请日：2013-03-13

申请人： The J. David Gladstone Institutes ,  Icahn School of Medicine at Mount Sinai

发明人： Melanie Ott ,  Eric M. Verdin ,  Ming-Ming Zhou

IPC分类号： A61K31/5517 ,  A61K31/4745 ,  G01N33/569 ,  A61K31/517 ,  A61K45/06 ,  A61K31/37 ,  A61K31/4184

CPC分类号： A61K31/5517 ,  A61K31/37 ,  A61K31/4184 ,  A61K31/4706 ,  A61K31/4745 ,  A61K31/517 ,  A61K31/551 ,  A61K45/06 ,  G01N33/56988 ,  G01N2333/16

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency vims. The methods generally involve contacting an HIV-infected cell in which HIV is latent with an agent that binds a bromodomain (BRD) in the cell. Latently infected cells contain replication-competent integrated HIV-1 genomes that are blocked at the transcriptional level, resulting in the absence of viral protein expression. The present disclosure provides methods for reducing the reservoir of latent immunodeficiency virus in an individual.

摘要翻译： 本公开提供了重新激活潜在免疫缺陷病毒的组合物和方法。 所述方法通常包括将HIV潜在的HIV感染细胞与结合细胞中的溴结构域（BRD）的试剂接触。 潜在感染的细胞含有可复制的综合HIV-1基因组，其在转录水平被阻断，导致不存在病毒蛋白质表达。 本公开提供了减少个体中潜在免疫缺陷病毒的储库的方法。

公开(公告)号：US20220193209A1

公开(公告)日：2022-06-23

申请号：US17424453

申请日：2020-01-16

申请人： The J. David Gladstone institutes, a testamentary trust established under the Will of j. David Glads

发明人： Albert Vallejo Gracia ,  Emilie Besnard ,  Melanie Ott ,  Eric Verdin

IPC分类号： A61K38/55 ,  A61K45/06 ,  A61P31/18

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection.

公开(公告)号：US20140030295A1

公开(公告)日：2014-01-30

申请号：US13717041

申请日：2012-12-17

申请人： THE J. DAVID GLADSTONE INSTITUTES

发明人： Hyungwook Lim ,  Melanie Ott ,  Eric M. Verdin ,  Hye-Sook Kwon

IPC分类号： A61K39/39 ,  A61K38/21 ,  A61K38/28 ,  A61K45/06

CPC分类号： A61K39/39 ,  A61K31/00 ,  A61K31/05 ,  A61K31/4188 ,  A61K38/212 ,  A61K38/28 ,  A61K45/06

摘要： The present disclosure provides a method of increasing an immune response in an individual, the method involving administering to an individual in need thereof an inhibitor of SIRT1. The present disclosure provides a method of reducing an immune response, e.g., to treat chronic immune hyperactivity, the method generally involving administering to an individual in need thereof an activator of SIRT1. The present disclosure provides a method of modulating activation and differentiation of CD4+ T cells.

摘要翻译： 本公开提供了增加个体的免疫应答的方法，所述方法涉及向有需要的个体施用SIRT1抑制剂。 本公开提供了减少免疫应答的方法，例如治疗慢性免疫多动症，所述方法通常涉及向有需要的个体施用SIRT1的激活剂。 本公开提供了调节CD4 + T细胞的激活和分化的方法。

公开(公告)号：US20180002699A1

公开(公告)日：2018-01-04

申请号：US15488212

申请日：2017-04-14

申请人： The J. David Gladstone Institutes

发明人： Melanie Ott ,  Daniela Boehm

IPC分类号： C12N15/113 ,  A61K31/551 ,  A61K31/167 ,  A61K31/713 ,  A61K31/222

CPC分类号： C12N15/1137 ,  A61K31/167 ,  A61K31/19 ,  A61K31/22 ,  A61K31/222 ,  A61K31/365 ,  A61K31/551 ,  A61K31/713 ,  A61K38/15 ,  A61K45/06 ,  C12N2310/14 ,  C12N2310/531 ,  A61K2300/00

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus and/or reducing transcription of HIV integrated into the genome of an HIV-infected cell. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection.

公开(公告)号：US20170209460A1

公开(公告)日：2017-07-27

申请号：US15324561

申请日：2015-07-07

申请人： The J. David Gladstone Institutes ,  The Regents of the University of California

发明人： Melanie Ott ,  Andrew S. Kondratowicz ,  Nevan John Krogan ,  Priya Shirish Shah ,  Krystal Ann Fontaine

IPC分类号： A61K31/5513 ,  A61K31/444 ,  A61K31/506 ,  A61K9/00 ,  A61K31/5377

CPC分类号： A61K31/5513 ,  A61K9/0019 ,  A61K9/0053 ,  A61K31/18 ,  A61K31/381 ,  A61K31/444 ,  A61K31/506 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/551 ,  A61P31/14 ,  Y02A50/385

摘要： The present disclosure provides methods of treating a flavivirus infection, and compositions for use in the methods.

公开(公告)号：US20240295544A1

公开(公告)日：2024-09-05

申请号：US18022428

申请日：2021-08-24

申请人： The J. David Gladstone Institutes, a Testamentary Trust Established Under the Will of J. David Glads

发明人： Todd C. McDevitt ,  Bruce Conklin ,  Melanie Ott ,  Juan Perez-Bermejo ,  Michael Sungwon Kang ,  Sarah Rockwood ,  Camille Simoneau ,  Gokul Ramadoss ,  David Joy

IPC分类号： G01N33/50 ,  C12N5/077 ,  G01N33/68

CPC分类号： G01N33/5061 ,  C12N5/0657 ,  G01N33/5035 ,  G01N33/6887 ,  C12N2503/02 ,  C12N2506/45 ,  C12N2510/00 ,  G01N2333/165

摘要： Provided herein are methods and compositions useful for identifying compounds that can inhibit SARS-CoV-2 infection or the effects thereof, especially in cardiomyocytes (CMs), which are highly infectible by SARS-CoV-2 corona viruses.

公开(公告)号：US20210348243A1

公开(公告)日：2021-11-11

申请号：US17206020

申请日：2021-03-18

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads ,  The Regents of the University of California

发明人： Melanie Ott ,  Parinaz Fozouni ,  Jennifer A. Doudna ,  Daniel A. Fletcher ,  David Savage ,  Emeric Charles ,  Sungmin Son ,  Gagandeep Renuka Kumar ,  Neil Switz

IPC分类号： C12Q1/70 ,  C12Q1/6825 ,  C12N15/11 ,  C12N9/22

摘要： The present disclosure relates to methods using CRISPR-Cas13 enzyme, complexed with SARS-CoV-2 crRNA guide RNAs to detect and quantify the presence of SARS-CoV-2 RNA in a sample with enhanced specificity and sensitivity. These methods can be used to diagnose SARS-CoV-2 infection, quantify the concentration of SARS-CoV-2 RNA present in a sample, identify the presence of different SARS-CoV-2 splice variants, subtypes, or mutations, and to monitor reactivation of SARS-CoV-2 transcription.

公开(公告)号：US11098309B2

公开(公告)日：2021-08-24

申请号：US16422753

申请日：2019-05-24

申请人： The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone

发明人： Melanie Ott ,  Daniela Boehm

IPC分类号： A61K48/00 ,  C07H21/04 ,  C12N15/113 ,  A61K38/15 ,  A61K45/06 ,  A61K31/19 ,  A61K31/22 ,  A61K31/365 ,  A61K31/551 ,  A61K31/713 ,  A61K31/167 ,  A61K31/222

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus and/or reducing transcription of HIV integrated into the genome of an HIV-infected cell. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection.

公开(公告)号：US10351853B2

公开(公告)日：2019-07-16

申请号：US15488212

申请日：2017-04-14

申请人： The J. David Gladstone Institutes

发明人： Melanie Ott ,  Daniela Boehm

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  A61K38/15 ,  A61K45/06 ,  A61K31/19 ,  A61K31/22 ,  A61K31/365 ,  A61K31/551 ,  A61K31/713 ,  A61K31/167 ,  A61K31/222

摘要： The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus and/or reducing transcription of HIV integrated into the genome of an HIV-infected cell. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection.

公开(公告)号：US20240309473A1

公开(公告)日：2024-09-19

申请号：US18575944

申请日：2022-07-01

申请人： THE J. DAVID GLADSTONE INSTITUTES, A TESTAMENTARY TRUST ESTABLISHED UNDER THE WELL OF J. ,  REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Daniel Fletcher ,  Sungmin Son ,  Melanie Ott

IPC分类号： C12Q1/70 ,  C12Q1/6832

CPC分类号： C12Q1/701 ,  C12Q1/6832

摘要： Droplet detection of RNA enables quantification of the absolute amount of target RNA as well as identification of different variant or mutant RNA species. As described herein. RNA detection with high sensitivity and multiplexed specificity can be achieved with short detection times by encapsulating the Cas reaction in droplets and monitoring enzyme kinetics fluorescently.