公开(公告)号：US20150335718A1

公开(公告)日：2015-11-26

申请号：US14697797

申请日：2015-04-28

Applicant: The Johns Hopkins University

Inventor： David K. Karig

IPC: A61K38/45 ,  C09K3/00 ,  A61K36/06 ,  A61K35/12 ,  A61K35/74 ,  A61K36/18

CPC classification number: A61K38/45 ,  A61K35/12 ,  A61K35/63 ,  A61K35/74 ,  A61K36/06 ,  A61K36/064 ,  A61K36/18 ,  A61K36/899 ,  C09K3/00 ,  C12Y207/03002 ,  Y02A50/473 ,  A61K2300/00

Abstract: A method of preserving a cell-free protein expression system includes preserving a cell extract with a first non-reducing sugar alcohol to provide a preserved cell extract, preserving a reaction buffer with a second non-reducing sugar alcohol to provide a preserved reaction buffer, and preserving an energy source, so that the cell extract, the reaction buffer, and the energy source are preserved separately.

Abstract translation: 保存无细胞蛋白质表达系统的方法包括用第一非还原糖醇保存细胞提取物以提供保存的细胞提取物，用第二非还原性糖醇保留反应缓冲液以提供保存的反应缓冲液， 并保存能量源，使得细胞提取物，反应缓冲液和能量源分开保存。

公开(公告)号：US10260064B2

公开(公告)日：2019-04-16

申请号：US14669125

申请日：2015-03-26

Applicant: The Johns Hopkins University

Inventor： David K. Karig ,  Joshua T. Wolfe

IPC: C12N15/10

Abstract: A method of directed evolution screening includes selecting a protein expression platform for an evolution target, expressing a key rupture gene configured to trigger droplet rupture, developing gene regulatory circuits to control expression of the key rupture gene as a function of performance of the evolution target, encapsulating expression components in droplets, and triggering droplet rupture by expressing a rupture agent from the key rupture gene.

公开(公告)号：US09937239B2

公开(公告)日：2018-04-10

申请号：US14697797

申请日：2015-04-28

Applicant: The Johns Hopkins University

Inventor： David K. Karig

IPC: A61K38/00 ,  A61K38/45 ,  A61K35/74 ,  A61K36/18 ,  A61K36/06 ,  A61K35/12 ,  C09K3/00 ,  A61K36/899 ,  A61K36/064 ,  A61K35/63

CPC classification number: A61K38/45 ,  A61K35/12 ,  A61K35/63 ,  A61K35/74 ,  A61K36/06 ,  A61K36/064 ,  A61K36/18 ,  A61K36/899 ,  C09K3/00 ,  C12Y207/03002 ,  Y02A50/473 ,  A61K2300/00

Abstract: A method of preserving a cell-free protein expression system includes preserving a cell extract with a first non-reducing sugar alcohol to provide a preserved cell extract, preserving a reaction buffer with a second non-reducing sugar alcohol to provide a preserved reaction buffer, and preserving an energy source, so that the cell extract, the reaction buffer, and the energy source are preserved separately.

公开(公告)号：US20160281081A1

公开(公告)日：2016-09-29

申请号：US14669125

申请日：2015-03-26

Applicant: The Johns Hopkins University

Inventor： David K. Karig ,  Joshua T. Wolfe

IPC: C12N15/10

CPC classification number: C12N15/1058 ,  C12N15/1075

Abstract: A method of directed evolution screening includes selecting a protein expression platform for an evolution target, expressing a key rupture gene configured to trigger droplet rupture, developing gene regulatory circuits to control expression of the key rupture gene as a function of performance of the evolution target, encapsulating expression components in droplets, and triggering droplet rupture by expressing a rupture agent from the key rupture gene.

Abstract translation: 定向进化筛选的方法包括选择用于进化靶标的蛋白质表达平台，表达配置为触发液滴破裂的关键破裂基因，开发基因调控电路以控制关键破裂基因的表达作为进化靶标的性能的函数， 将表达组分包封在液滴中，并通过从关键破裂基因中表达破裂剂来触发液滴破裂。