公开(公告)号：US12227753B2

公开(公告)日：2025-02-18

申请号：US16755534

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85

Abstract: Provided are compositions and methods that include a CasY transactivating noncoding RNA (trancRNA) (referred to herein as a “CasY trancRNA”), nucleic acids encoding the CasY trancRNA, and/or a modified host cell comprising the CasY trancRNA (and/or a nucleic acid encoding the same). Subject compositions and methods can also include one or more of: (a) a “CasY” protein (also referred to as a CasY polypeptide, a Cas12d protein, and a Cas12d polypeptide), a nucleic acid encoding the CasY protein, and/or a modified host cell comprising the CasY protein (and/or a nucleic acid encoding the same); and (b) a CasY guide RNA (also referred to herein as a “Cas12d guide RNA”) that binds to and provides sequence specificity to the CasY protein, a nucleic acid encoding the CasY guide RNA, and/or a modified host cell comprising the CasY guide RNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200172886A1

公开(公告)日：2020-06-04

申请号：US16789049

申请日：2020-02-12

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20240301376A1

公开(公告)日：2024-09-12

申请号：US18618668

申请日：2024-03-27

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12N2310/20

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11441137B2

公开(公告)日：2022-09-13

申请号：US16884766

申请日：2020-05-27

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12Q1/6809 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200299660A1

公开(公告)日：2020-09-24

申请号：US16896711

申请日：2020-06-09

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11371031B2

公开(公告)日：2022-06-28

申请号：US17032521

申请日：2020-09-25

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12Q1/6809 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200339967A1

公开(公告)日：2020-10-29

申请号：US16757981

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/63

Abstract: Provided are compositions and methods that include one or more of: (1) a Cas12c protein (also referred to as a C2c3 protein), a nucleic acid encoding the Cas12c protein, and/or a modified host cell comprising the Cas12c protein (and/or a nucleic acid encoding the same); (2) a Cas12c guide RNA (also referred to herein as a C2c3 guide RNA) that binds to and provides sequence specificity to the Cas12c protein, a nucleic acid encoding the Cas12c guide RNA, and/or a modified host cell comprising the Cas12c guide RNA (and/or a nucleic acid encoding the same); and (3) a Cas12c transactivating noncoding RNA (trancRNA) (referred to herein as a Cas12c trancRNA or C2c3 trancRNA), a nucleic acid encoding the Cas12c trancRNA, and/or a modified host cell comprising the Cas12c trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200087640A1

公开(公告)日：2020-03-19

申请号：US16694720

申请日：2019-11-25

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20240167009A1

公开(公告)日：2024-05-23

申请号：US18393303

申请日：2023-12-21

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C07K2319/09 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11970719B2

公开(公告)日：2024-04-30

申请号：US16755535

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12N2310/20

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).