公开(公告)号：US20210002697A1

公开(公告)日：2021-01-07

申请号：US16856343

申请日：2020-04-23

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/686 ,  C12Q1/70 ,  C12Q1/6886 ,  C12Q1/689

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US10697007B2

公开(公告)日：2020-06-30

申请号：US15317393

申请日：2015-06-25

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/70 ,  C12Q1/6886 ,  C12Q1/689

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20220275427A1

公开(公告)日：2022-09-01

申请号：US17697502

申请日：2022-03-17

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/686 ,  C12Q1/70 ,  C12Q1/6886 ,  C12Q1/689

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20180237836A1

公开(公告)日：2018-08-23

申请号：US15753132

申请日：2016-08-16

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Freeman Lan ,  Shaun Lim ,  Angus Sidore

IPC: C12Q1/6827 ,  C12P19/34 ,  C12Q1/6844 ,  C40B30/06 ,  C12N9/12

CPC classification number: C12Q1/6827 ,  C12N9/1252 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2531/119 ,  C12Q2535/122 ,  C12Q2537/16 ,  C12Q2563/159 ,  C40B30/06

Abstract: Methods for non-specifically amplifying a nucleic acid template molecule are provided. The methods may be used to amplify nucleic acid template molecule(s) for sequencing, e.g., for sequencing the genomes of uncultivable microbes or sequencing to identify copy number variation in cancer cells. Aspects of the disclosed methods may include non-specifically amplifying a nucleic acid template molecule, including encapsulating in a microdroplet a nucleic acid template molecule obtained from a biological sample, introducing multiple displacement amplification (MDA) reagents and a plurality of MDA primers into the microdroplet, and incubating the microdroplet under conditions effective for the production of MDA amplification products, wherein the incubating is effective to produce MDA amplification products from the nucleic acid template molecule.

公开(公告)号：US11312990B2

公开(公告)日：2022-04-26

申请号：US16856343

申请日：2020-04-23

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/686 ,  C12Q1/70 ,  C12Q1/689 ,  C12Q1/6886

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20170121756A1

公开(公告)日：2017-05-04

申请号：US15317393

申请日：2015-06-25

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/70 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20170022538A1

公开(公告)日：2017-01-26

申请号：US15014976

申请日：2016-02-03

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Shaun Lim ,  David Sukovich ,  Shea Thompson Lance

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01F3/0807 ,  B01F5/0653 ,  B01F13/0062 ,  C12Q1/68 ,  C40B40/06 ,  C40B50/06 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2565/607 ,  C12Q2565/626

Abstract: Multiple-emulsion nucleic acid amplification allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the disclosure are also provided.

Abstract translation: 多重乳液核酸扩增允许根据用核酸扩增技术（例如PCR）检测的序列检测，定量和/或分选生物系统中所含的核酸。 核酸可以自由漂浮或包含在生物或非生物结构中，包括颗粒，病毒和细胞。 核酸可以包括例如DNA或RNA。 还提供了用于本公开的实践方法的系统和设备。