公开(公告)号：US20230212646A1

公开(公告)日：2023-07-06

申请号：US17928198

申请日：2021-05-27

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Linfeng Xu ,  Leqian Liu ,  Kai-chun Chang

IPC: C12Q1/6806 ,  C12Q1/6827 ,  B01L3/00 ,  C12Q1/686

CPC classification number: C12Q1/6806 ,  C12Q1/6827 ,  B01L3/502784 ,  B01L3/502715 ,  C12Q1/686 ,  C12Q2600/156 ,  B01L2300/0816 ,  B01L2200/16 ,  B01L2300/0861 ,  B01L2300/0636 ,  B01L2200/027

Abstract: Provided is a method including encapsulating a biological material in a droplet having a volume of 500 nl or less, depositing the droplet to an addressable location of a substrate, and performing mass spectroscopy on the droplet. The method can further include conducting omic analysis on the droplet, such as sequencing DNA or analyzing mRNA, after the mass spectroscopy. In some cases, the method can be used to screen thousands of genetically different cells to identify correlations between genetics and the production of a metabolite, wherein the metabolite is detected by mass spectroscopy. Also provided is a system for performing the method.

公开(公告)号：US20210388446A1

公开(公告)日：2021-12-16

申请号：US17223565

申请日：2021-04-06

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12Q1/6886 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  B01F5/06 ,  B01F13/00 ,  B01F3/08 ,  C12Q1/686 ,  C12Q1/6844

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US20210002697A1

公开(公告)日：2021-01-07

申请号：US16856343

申请日：2020-04-23

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/686 ,  C12Q1/70 ,  C12Q1/6886 ,  C12Q1/689

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20150232942A1

公开(公告)日：2015-08-20

申请号：US14420646

申请日：2013-08-12

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastbum ,  Adam R. Sciambi

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01F3/0807 ,  B01F5/0652 ,  B01F13/0062 ,  B01F13/0076 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2537/143 ,  C12Q2565/629

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

Abstract translation: 提供了从生物样品中检测组分的方法。 在某些方面，所述方法可用于检测和/或定量生物样品中的特定组分，例如肿瘤细胞（例如循环肿瘤细胞）。 还提供了用于实践主题方法的系统和设备。

公开(公告)号：US20240175091A1

公开(公告)日：2024-05-30

申请号：US18544232

申请日：2023-12-18

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12Q1/6886 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01F33/3031 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686

CPC classification number: C12Q1/6886 ,  B01F23/41 ,  B01F25/4335 ,  B01F33/3011 ,  B01F33/3031 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US11891666B2

公开(公告)日：2024-02-06

申请号：US17223565

申请日：2021-04-06

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12P19/34 ,  C12Q1/6886 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01F33/3031 ,  C12Q1/686 ,  C12Q1/6844

CPC classification number: C12Q1/6886 ,  B01F23/41 ,  B01F25/4335 ,  B01F33/3011 ,  B01F33/3031 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6844 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US11203787B2

公开(公告)日：2021-12-21

申请号：US16164707

申请日：2018-10-18

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12P19/34 ,  C12Q1/6886 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  B01F5/06 ,  B01F13/00 ,  B01F3/08 ,  C12Q1/686 ,  C12Q1/6844

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US11142791B2

公开(公告)日：2021-10-12

申请号：US16324532

申请日：2017-08-09

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  David Sukovich

IPC: C12Q1/686 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6886 ,  C12M3/00 ,  C12Q1/68 ,  B01L7/00 ,  B01L3/00

Abstract: The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.

公开(公告)号：US11020736B2

公开(公告)日：2021-06-01

申请号：US16552982

申请日：2019-08-27

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Adam R. Sciambi ,  Russell Cole ,  Zev Jordan Gartner

IPC: B01L3/02 ,  B01L3/00 ,  G01N15/14 ,  G01N15/10 ,  G01N35/10

Abstract: Methods for delivering discrete entities including, e.g., cells, media or reagents to substrates are provided. In certain aspects, the methods include manipulating and/or analyzing qualities of the entities or biological components thereof. In some embodiments, the methods may be used to create arrays of microenvironments and/or for two and three-dimensional printing of tissues or structures. Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US20200261879A1

公开(公告)日：2020-08-20

申请号：US16649068

申请日：2018-09-28

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Makiko N. Hatori ,  Leqian Liu ,  Samuel Kim ,  Cyrus Modavi

IPC: B01J13/08 ,  C12Q1/6869 ,  C12Q1/6844 ,  B01F17/00

Abstract: The methods described herein, referred to as particle-templated emulsification (PTE), provide an improved approach for generating a monodisperse emulsion that encapsulates target particles of interest without requiring the use of a microfluidic device. Monodisperse droplets may be effectively obtained by using monodisperse particles to template the formation of droplets, which can include, e.g., monodisperse single-emulsion droplets, multiple-emulsion droplets, or Giant Unilamellar Vesicles (GUV), without destroying the integrity of the droplets.