公开(公告)号：US10988805B2

公开(公告)日：2021-04-27

申请号：US15552040

申请日：2016-02-19

申请人： The Regents of the University of California

发明人： Sriram Kosuri ,  Rocky Cheung ,  Nathan B. Lubock

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12N15/10

摘要： In some aspects, the invention relates to a method for producing a barcoded DNA library, comprising: optionally fragmenting DNA to produce fragmented DNA; combining the DNA/fragments with control nucleotide sequences to produce a mixture of nucleic acids, each control nucleotide sequence comprising a variable nucleotide sequence, thereby barcoding the DNA/fragments; amplifying the mixture of nucleic acids to produce amplified nucleic acids; isolating a plurality of nucleic acids from the amplified nucleic acids; and rearranging the nucleic acids of the plurality, thereby producing the barcoded DNA library.

公开(公告)号：US20180037951A1

公开(公告)日：2018-02-08

申请号：US15552040

申请日：2016-02-19

申请人： The Regents of the University of California

发明人： Sriram Kosuri ,  Rocky Cheung ,  Nathan B. Lubock

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12N15/1082 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/185 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2531/125

摘要： In some aspects, the invention relates to a method for producing a barcoded DNA library, comprising: optionally fragmenting DNA to produce fragmented DNA; combining the DNA/fragments with control nucleotide sequences to produce a mixture of nucleic acids, each control nucleotide sequence comprising a variable nucleotide sequence, thereby barcoding the DNA/fragments; amplifying the mixture of nucleic acids to produce amplified nucleic acids; isolating a plurality of nucleic acids from the amplified nucleic acids; and rearranging the nucleic acids of the plurality, thereby producing the barcoded DNA library.