公开(公告)号：US20210003581A1

公开(公告)日：2021-01-07

申请号：US16316127

申请日：2017-08-07

申请人： The Texas A&M University System

发明人： Gerard L. COTE ,  Javier T. GARZA

IPC分类号： G01N33/58 ,  G01N33/543 ,  G01N21/65

摘要： Provided are methods of quantifying analyte concentration in a sample by providing a pre-screened amount of an enzyme that has been pre-screened against the analyte in the sample; associating the pre-screened enzyme with a Raman reporter molecule that reacts with the enzyme to result in a change in the Raman spectrum pattern of the Raman reporter molecule; detecting the change in the Raman spectrum pattern of the Raman reporter molecule; and correlating the change (e.g., change rate) to the concentration of the analyte in the sample. Pre-screening may affect the concentration or activity of the enzyme in proportion to analyte concentration. Correlation may occur by correlating the change in the Raman spectrum pattern of the Raman reporter molecule to the concentration of the pre-screened amount of the enzyme, and correlating the concentration of the pre-screened amount of the enzyme to the concentration of the analyte in the sample.