公开(公告)号：US20250034621A1

公开(公告)日：2025-01-30

申请号：US18782100

申请日：2024-07-24

Applicant: The Texas A&M University System

Inventor： Sayantan TRIPATHY ,  Samuel MABBOTT ,  Gerard L. COTE

IPC: C12Q1/682 ,  G01N33/543 ,  G01N33/68

Abstract: In one aspect, the disclosure relates to a universal method for biomarker extraction and detection from clinical samples using magnetic nanoparticles (MNPs) coupled with a locked aptamer-initiator approach. The MNP bound locked aptamer undergoes a conformational change in the presence of the target biomarker and the unfolded initiator triggers a hybridization chain reaction with a structurally stabilized catalytic trimeric triplex DNAzyme hairpin. Amplicons from the hybridization chain reaction can be incubated with hemin to obtain a stable triplex structure that has horseradish peroxidase-like activity in the presence of H2O2 and a substrate molecule. The intensity of the signal is directly proportional to the amount of biomarker present. Binding one or more reagents on a nanoparticle can be coupled with this approach for use in a microfluidic or paper fluidic platform. In any of these aspects, the assay can detect as low as femtogram amounts of biomarker from the clinical sample.